| IED ID | IndEnz0015000126 |
| Enzyme Type ID | laccase000126 |
| Protein Name |
Laccase-1 Lac1 EC 1.10.3.2 Benzenediol:oxygen oxidoreductase 1 Diphenol oxidase 1 Urishiol oxidase 1 Fragments |
| Gene Name | |
| Organism | Galerina sp. |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Strophariaceae Galerina unclassified Galerina Galerina sp. |
| Enzyme Sequence | GPSTDLVIGNKGFDGGIDSAILR |
| Enzyme Length | 23 |
| Uniprot Accession Number | P86351 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by sodium azide, sodium cyanide, Li(+), Sn(+), Hg(2+), and the disulfide-reducing agents beta-mercaptoethanol, dithiothreitol and thioglycolic acid. Moderately inhibited by Mn(2+) and Fe(2+), inhibition by these metal ions is stronger at 0.1 mM than at 1 mM. Moderately inhibited by Cu(2+). {ECO:0000269|Ref.1}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000269|Ref.1}; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products. Demethylates eucalyptus hard wood lignin. Has high activity against the non-phenolic heterocyclic compound ABTS, and lower activity against the phenolic substrates syringic acid, caffeic acid, syringaldazine, vanillic acid, catechol and levodihydroxyphenylalanine. {ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. Shows 50% of its maximal activity at 20 degrees Celsius. Has a half-life of 53 minutes at 50 degrees Celsius, 23 minutes at 60 degrees Celsius, and less than 2 minutes at 70 degrees Celsius. {ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 3.0 with ABTS as substrate, activity decreases sharply as pH increases, and no activity is seen at pH 7.0. Optimum pH is 4.0 with syringic acid as substrate, 5.0 with caffeic acid, syringaldazine, vanillic acid or catechol as substrate, and 6.0 with levodihydroxyphenylalanine as substrate. Inactive after 3 hours incubation at pH 2.0, retains 20% of its activity after 24 hours at pH 3.0 and 90% of its activity after 24 hours at pH 9.0. {ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-adjacent residues (1); Non-terminal residue (2) |
| Keywords | Copper;Direct protein sequencing;Lignin degradation;Metal-binding;Oxidoreductase;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,303 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=14 uM for ABTS {ECO:0000269|Ref.1}; KM=0.8 uM for syringaldazine {ECO:0000269|Ref.1}; |
| Metal Binding | |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |