| IED ID | IndEnz0015000131 |
| Enzyme Type ID | laccase000131 |
| Protein Name |
Laccase EC 1.10.3.2 Fragment |
| Gene Name | |
| Organism | Sanghuangporus baumii (Phellinus baumii) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Hymenochaetales Hymenochaetaceae Sanghuangporus Sanghuangporus baumii (Phellinus baumii) |
| Enzyme Sequence | AIGPVDEV |
| Enzyme Length | 8 |
| Uniprot Accession Number | C0HJB2 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by Ca(2+), Fe(2+) and Al(3+). {ECO:0000269|Ref.1}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000269|Ref.1}; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products. Exhibits broad substrate specificity against a range of aromatic compounds with highest activity against guaiacol. Shows lower activity against 4-methylcatechol, 4-hydroxyindole, catechol, hydroquinone and 2,6-dimethoxy-phenol. {ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 20 degrees Celsius. Thermostable, maintains activity from 50 to 60 degrees Celsius. {ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 2.4. Stable at pH 7.2 and 5.2. {ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Lignin degradation;Oxidoreductase;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 799 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |