| IED ID | IndEnz0015000138 |
| Enzyme Type ID | laccase000138 |
| Protein Name |
Laccase EC 1.10.3.2 Benzenediol:oxygen oxidoreductase Diphenol oxidase Urishiol oxidase Fragment |
| Gene Name | |
| Organism | Hericium coralloides (Coral tooth fungus) (Hericium ramosum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Russulales Hericiaceae Hericium Hericium coralloides (Coral tooth fungus) (Hericium ramosum) |
| Enzyme Sequence | AVGDDTPQLY |
| Enzyme Length | 10 |
| Uniprot Accession Number | B3A0L4 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Strongly activated by Mg(2+) and Al(3+). At concentrations <50 mM, activated by Ca(2+), Mn(2+), Co(2+) and K(+). Strongly inhibited by Hg(2+) and, in a concentration-dependent manner, by Fe(2+). {ECO:0000269|PubMed:22367940}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000269|PubMed:22367940}; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products. Has activity towards ABTS and, to a much lesser extent, towards N,N-dimethyl-1,4-phenylenediamine, catechol and 2-methylcatechol. {ECO:0000269|PubMed:22367940}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius. Retains 65% activity at 20 degrees Celsius and 24% activity at 100 degrees Celsius. {ECO:0000269|PubMed:22367940}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 2.2. Activity declines sharply with decreases or increases in pH and is absent above pH 6.6. {ECO:0000269|PubMed:22367940}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Copper;Direct protein sequencing;Lignin degradation;Metal-binding;Oxidoreductase;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:D0VWU3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,078 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |