Detail Information for IndEnz0015000149
IED ID IndEnz0015000149
Enzyme Type ID laccase000149
Protein Name Trihydroxynaphthalene reductase PfmaI
EC 1.1.1.-
Conidial pigment biosynthesis protein I
Gene Name Pfmaj PFICI_05460
Organism Pestalotiopsis fici (strain W106-1 / CGMCC3.15140)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Xylariomycetidae Xylariales Sporocadaceae Pestalotiopsis Pestalotiopsis fici Pestalotiopsis fici (strain W106-1 / CGMCC3.15140)
Enzyme Sequence MPGVTSERTASRFDAIPGPLGLASASLEGKVALVTGAGRGIGREMAMELGRRGAKVIVNYANSSESAQEVVNAIKKSGSDAVAIKANVSDVDQIVSLFDQAVKVWGKLHIVCSNSGVVSFGHVKDVTPEEFDRVFTINTRGQFFVAREAYKHLEVGGRLILMGSITGQAKGVPKHAVYSGSKGAIETFVRCMAVDFGDKKITVNAVAPGGIKTDMYHAVCREYIPGGTELDDEAVDEYASTWSPLGRVGLPIDIARCVCFLASQDGEWVNGKVLGIDGHAMM
Enzyme Length 282
Uniprot Accession Number W3XC32
Absorption
Active Site ACT_SITE 178; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q92506
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 1.1.1.-
Enzyme Function FUNCTION: Trihydroxynaphthalene reductase involved the biosynthesis of dihydroxynaphthalene (DHN)-melanin, a bluish-green pigment forming a dark layer in the conidial wall that protects the conidia from UV radiations (PubMed:28517364). The first step of the pathway is the production of the pentaketide 1,3,6,8-tetrahydroxynaphthalene (1,3,6,8-THN or T4HN) by the polyketide synthase PfmaE though condensation of acetyl-CoA with malonyl-CoA. T4HN is not stable and easily oxidizes into the stable form flaviolin (PubMed:28517364). T4HN is also substrate of the hydroxynaphthalene reductase PfmaG to yield scytalone (PubMed:28517364). The scytalone dehydratase PfmaJ then reduces scytalone to 1,3,8-THN (PubMed:31116900). 1,3,8-THN is then substrate of the hydroxynaphthalene reductase PfmaI to yield vermelone (Probable). Vermelone is further converted by the multicopper oxidase PfmaD to 1,8-DHN (Probable). Finally the laccase PFICI_06862 transforms 1,8-DHN to DHN-melanin (Probable). The roles of the 5-oxoprolinase PfmaA and the proline iminopeptidase PfmaB within the cluster have not been elucidated yet (Probable). {ECO:0000269|PubMed:28517364, ECO:0000269|PubMed:31116900, ECO:0000305|PubMed:28517364, ECO:0000305|PubMed:31116900}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Pigment biosynthesis; melanin biosynthesis. {ECO:0000305|PubMed:31116900}.
nucleotide Binding NP_BIND 33..41; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 61..62; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 86..88; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 178..182; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 211..213; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506
Features Active site (1); Chain (1); Nucleotide binding (5)
Keywords Melanin biosynthesis;NADP;Oxidoreductase;Reference proteome
Interact With
Induction INDUCTION: Expression is positively regulated by the cluster-specific transcription factor PfmaF. {ECO:0000269|PubMed:28517364, ECO:0000269|PubMed:31116900}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 29,907
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda