| IED ID |
IndEnz0016000003 |
| Enzyme Type ID |
tyrosinase000003 |
| Protein Name |
Tyrosinase
EC 1.14.18.1
Albino locus protein
Monophenol monooxygenase
|
| Gene Name |
Tyr |
| Organism |
Mus musculus (Mouse) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Glires (Rodents and rabbits)
Rodentia
Myomorpha (mice and others)
Muroidea
Muridae
Murinae
Mus
Mus
Mus musculus (Mouse)
|
| Enzyme Sequence |
MFLAVLYCLLWSFQISDGHFPRACASSKNLLAKECCPPWMGDGSPCGQLSGRGSCQDILLSSAPSGPQFPFKGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFGGPNCTEKRVLIRRNIFDLSVSEKNKFFSYLTLAKHTISSVYVIPTGTYGQMNNGSTPMFNDINIYDLFVWMHYYVSRDTLLGGSEIWRDIDFAHEAPGFLPWHRLFLLLWEQEIRELTGDENFTVPYWDWRDAENCDICTDEYLGGRHPENPNLLSPASFFSSWQIICSRSEEYNSHQVLCDGTPEGPLLRNPGNHDKAKTPRLPSSADVEFCLSLTQYESGSMDRTANFSFRNTLEGFASPLTGIADPSQSSMHNALHIFMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLLEVYPEANAPIGHNRDSYMVPFIPLYRNGDFFITSKDLGYDYSYLQESDPGFYRNYIEPYLEQASRIWPWLLGAALVGAVIAAALSGLSSRLCLQKKKKKKQPQEERQPLLMDKDDYHSLLYQSHL |
| Enzyme Length |
533 |
| Uniprot Accession Number |
P11344 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; Evidence={ECO:0000269|PubMed:1537333}; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; Evidence={ECO:0000269|PubMed:1537333}; |
| DNA Binding |
|
| EC Number |
1.14.18.1 |
| Enzyme Function |
FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (PubMed:2517217). Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (PubMed:2517217, PubMed:1537333). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:1537333). {ECO:0000269|PubMed:1537333, ECO:0000269|PubMed:2517217}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Glycosylation (6); Metal binding (6); Natural variant (3); Sequence caution (1); Sequence conflict (4); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords |
Albinism;Copper;Disease variant;Glycoprotein;Melanin biosynthesis;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix |
| Interact With |
P07147 |
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Melanosome membrane {ECO:0000250|UniProtKB:P14679}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P14679}. Melanosome {ECO:0000269|PubMed:26620560}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. {ECO:0000269|PubMed:26620560}. |
| Modified Residue |
|
| Post Translational Modification |
PTM: Glycosylated. {ECO:0000269|PubMed:1537333}. |
| Signal Peptide |
SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
10068480;
10087302;
10212311;
10224267;
10325425;
1033984;
10348635;
10352232;
10369930;
10431243;
10441734;
10485648;
10491617;
10501973;
10556422;
10583387;
10585447;
10588862;
10596759;
10625389;
1063212;
10640708;
10665936;
10677587;
10748239;
10760285;
10852913;
10890980;
1094920;
10995574;
10998130;
11008180;
11054672;
11095635;
11105050;
11110696;
11209059;
11214319;
11217851;
11236829;
11238064;
11247670;
11254699;
11266470;
11277491;
11309660;
1133851;
11391006;
11454769;
11455427;
11474208;
11493524;
11523704;
11532192;
11587854;
11641724;
11730008;
11839810;
11917121;
11925014;
12028586;
12040030;
12058062;
12100493;
12100494;
12215665;
1225761;
12412141;
12427839;
12466851;
12624268;
12666202;
12724309;
1278263;
1280558;
12829739;
12832289;
12899938;
1292010;
1292011;
1292012;
12955145;
1303276;
1349573;
1349842;
1352884;
13545569;
1354647;
13599016;
1360278;
1362181;
1365899;
1378547;
13877379;
13880466;
13914573;
13955956;
14051862;
1409439;
1409440;
1409443;
1409445;
14170399;
1427844;
1427845;
14576318;
14595836;
14622170;
14739285;
14990867;
1507224;
15161869;
15161941;
1535334;
1540161;
1543902;
1543912;
15459106;
15477596;
15489520;
15572362;
1557387;
15576400;
15618488;
15722110;
1572647;
15760341;
15826307;
15978262;
15983061;
16141072;
16146766;
16162817;
1617218;
16180137;
1618394;
16185282;
16199032;
16261419;
16303920;
1638084;
16384915;
1644288;
16602821;
16684884;
1671854;
1673105;
1679745;
1679753;
1680807;
16827931;
1684331;
16847266;
16879986;
16899383;
16988713;
17024248;
1706745;
17083330;
17196956;
17202262;
17245945;
172460;
17246029;
17246039;
17246054;
17246260;
17247190;
17247200;
17247280;
17247639;
17291453;
1741389;
1744088;
17516930;
17541153;
17541167;
17617591;
17805301;
17850513;
18042623;
18083586;
18117146;
18177347;
18199537;
1822431;
18237444;
18272592;
18344445;
1834548;
18378571;
18385071;
18425346;
18469339;
18568886;
18606814;
18625748;
18641339;
18650808;
18655794;
18715234;
18890162;
1889814;
18983533;
1900251;
19098321;
1912584;
19141317;
19155314;
19168892;
19220779;
19299493;
19324852;
19351821;
19456252;
19493314;
1952626;
19661232;
1968044;
1970330;
1970795;
1981054;
19855129;
2004758;
20053998;
20130004;
2040451;
20444203;
2052619;
20685971;
2077114;
20876567;
208984;
21159798;
21191399;
2119500;
21211561;
21212186;
2124349;
21337463;
21364941;
2137109;
21397665;
21410935;
21494554;
21625383;
21667118;
21677750;
21703427;
21791610;
21943097;
21968110;
22094257;
22242171;
22256768;
22275436;
22291038;
22474390;
22492037;
22540041;
22561987;
2264930;
22711892;
22792072;
22973003;
22993390;
2300582;
2304907;
2307474;
23123854;
23389444;
2344351;
23526936;
23620107;
23708969;
2378752;
23844102;
23898044;
24124512;
24355922;
24440150;
24503860;
24562306;
24599260;
24769727;
2478463;
24875170;
24876386;
24879364;
24884952;
24885435;
24950377;
25007044;
25236450;
25273269;
25368159;
2541065;
2552132;
25587897;
25713930;
25769612;
25857772;
25957904;
25972253;
26023234;
26048593;
26060116;
26272364;
26283863;
26339033;
26656104;
26876013;
269;
2706836;
27097562;
27224051;
273256;
2744450;
27460988;
27488639;
27526711;
27804998;
27892516;
27974362;
28040780;
2806116;
2813427;
28203742;
2823263;
2846281;
2859594;
28614705;
28760957;
28778995;
28835637;
29167567;
293741;
29466759;
29720086;
3008521;
30254141;
30273664;
30448402;
30590377;
31201094;
31260037;
31269433;
31418010;
31428695;
3146546;
3181757;
31924792;
32313931;
32797202;
32986721;
3319884;
33290311;
33484482;
3378451;
34380032;
34453947;
3468297;
34702932;
34703992;
34750345;
34941868;
35041655;
3582592;
3596986;
36985;
3839762;
3857594;
3866909;
3899099;
3971387;
4020956;
4141683;
4150768;
4296364;
4343696;
4397868;
447069;
447675;
4634048;
4747916;
4822986;
4823665;
4823666;
4841974;
4842093;
4920403;
5005683;
521742;
522864;
5364923;
536710;
540025;
5424503;
549645;
5527659;
5558564;
5565073;
568581;
574104;
5902512;
6062374;
6088946;
6162796;
621381;
62358;
6276492;
62795;
6310574;
6327825;
6328281;
6572398;
6579548;
6585792;
6585968;
6706951;
6785376;
6793688;
6802749;
68973;
690964;
7028029;
7117820;
7117836;
7161508;
7262555;
7479744;
7504460;
7558026;
7608332;
7665913;
7681531;
7688401;
7712116;
7767008;
7774558;
7774957;
7806222;
7813769;
7846052;
7849740;
7852195;
7873577;
7892256;
7896292;
7920633;
7949739;
7961973;
7963676;
8039502;
8040269;
8040609;
8094303;
8098217;
8099513;
8114702;
8129415;
8197131;
8200511;
8223273;
8227355;
826246;
8278937;
8307326;
8307327;
8390964;
8422502;
8432998;
8432999;
8433000;
8433007;
8459851;
8482578;
8499946;
8504252;
8505373;
8570653;
8575777;
8601447;
8626800;
8631264;
863237;
8636124;
8654502;
8661014;
8672126;
8747923;
8747928;
8783939;
8786140;
8787751;
8812411;
8812504;
8833147;
8838319;
8875950;
8884266;
8898882;
8901055;
8903730;
8921397;
8947025;
9008228;
9023352;
9088570;
9144237;
9168903;
9207131;
9305902;
9335613;
93520;
9417870;
9477059;
9510032;
9548375;
9636156;
9666083;
9680387;
9689118;
9722945;
9722956;
9799267;
9808625;
9822646;
9841903;
9880655;
9918801;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
60,606 |
| Kinetics |
|
| Metal Binding |
METAL 180; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 202; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 211; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 363; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 367; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 390; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19 |
| Rhea ID |
RHEA:34287; RHEA:18117 |
| Cross Reference Brenda |
1.14.18.1; |