| IED ID | IndEnz0016000005 |
| Enzyme Type ID | tyrosinase000005 |
| Protein Name |
5,6-dihydroxyindole-2-carboxylic acid oxidase DHICA oxidase EC 1.14.18.- Brown locus protein Catalase B Tyrosinase-related protein 1 TRP TRP-1 TRP1 |
| Gene Name | Tyrp1 Tyrp-1 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MKSYNVLPLAYISLFLMLFYQVWAQFPRECANIEALRRGVCCPDLLPSSGPGTDPCGSSSGRGRCVAVIADSRPHSRHYPHDGKDDREAWPLRFFNRTCQCNDNFSGHNCGTCRPGWRGAACNQKILTVRRNLLDLSPEEKSHFVRALDMAKRTTHPQFVIATRRLEDILGPDGNTPQFENISVYNYFVWTHYYSVKKTFLGTGQESFGDVDFSHEGPAFLTWHRYHLLQLERDMQEMLQEPSFSLPYWNFATGKNVCDVCTDDLMGSRSNFDSTLISPNSVFSQWRVVCESLEEYDTLGTLCNSTEGGPIRRNPAGNVGRPAVQRLPEPQDVTQCLEVRVFDTPPFYSNSTDSFRNTVEGYSAPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNADISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYAYEVQWPGQEFTVSEIITIAVVAALLLVAAIFGVASCLIRSRSTKNEANQPLLTDHYQRYAEDYEELPNPNHSMV |
| Enzyme Length | 537 |
| Uniprot Accession Number | P07147 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.14.18.- |
| Enzyme Function | FUNCTION: Plays a role in melanin biosynthesis (PubMed:2245916). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid (PubMed:7813420). May regulate or influence the type of melanin synthesized (PubMed:7813420, PubMed:2245916). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (PubMed:1537333). {ECO:0000269|PubMed:1537333, ECO:0000269|PubMed:2245916, ECO:0000269|PubMed:7813420}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis; melanin biosynthesis. {ECO:0000269|PubMed:2245916}. |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (7); Glycosylation (6); Metal binding (6); Natural variant (2); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | 3D-structure;Albinism;Copper;Disease variant;Disulfide bond;Glycoprotein;Melanin biosynthesis;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc |
| Interact With | P11344 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Melanosome membrane {ECO:0000269|PubMed:17182842, ECO:0000269|PubMed:18650808}; Single-pass type I membrane protein {ECO:0000269|PubMed:17182842}. Melanosome {ECO:0000269|PubMed:26620560}. Note=Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. {ECO:0000269|PubMed:17182842, ECO:0000269|PubMed:26620560}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:1537333}. |
| Signal Peptide | SIGNAL 1..24 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 6MP0; 6MP1; |
| Mapped Pubmed ID | 10049573; 10068480; 10087289; 10087299; 10087302; 10191100; 10192392; 102659; 10337627; 10373328; 10395806; 10452857; 10491617; 10517996; 10602994; 10604301; 10644000; 10946246; 10978518; 11178107; 11266470; 11277491; 1133851; 11526516; 11743578; 11829488; 11917121; 12140685; 12183685; 12205107; 12644183; 1280558; 12829739; 12893462; 1292007; 1303241; 1318721; 1321344; 1352157; 1352884; 1371941; 1378547; 1379092; 13877379; 13880466; 1398056; 14009199; 14047421; 1409444; 1409609; 14098314; 1421760; 1450512; 14681479; 15161859; 15415586; 15520276; 15576400; 15707965; 15728386; 15985430; 16038893; 16103918; 16141072; 16162817; 16185282; 16219534; 16247030; 1630810; 16332269; 1639403; 16505033; 16505357; 16783635; 16827931; 16837549; 1684952; 1686571; 16934245; 1704135; 1706745; 17247190; 17247200; 17247280; 17247639; 1748176; 1756209; 17608931; 17619104; 1769662; 17724166; 18042623; 18117146; 18158332; 18354038; 1836514; 18402690; 18715234; 1871601; 1907365; 1908461; 19293329; 19403694; 19493314; 1952626; 19561540; 196096; 1978328; 19855129; 19879162; 20123016; 20156971; 20156973; 2037292; 20460369; 20633051; 20668223; 2072972; 20739468; 21187289; 21236676; 21267068; 21289306; 21350176; 2141254; 21677750; 21757588; 21833020; 21949658; 21976956; 2212703; 221612; 22275436; 2245917; 22556244; 22705010; 2303035; 2304907; 23203930; 23322566; 23370329; 23389444; 23523356; 23536636; 2354437; 23549422; 2379821; 23999499; 2400934; 24550361; 24769727; 24875170; 24884952; 25118265; 25236450; 2568329; 25872655; 2591966; 26472242; 26876013; 27011731; 27072778; 27390154; 27857681; 2850257; 28684211; 28981549; 29129787; 29370175; 29602773; 29760184; 29894691; 2993652; 2997987; 30273664; 30347088; 30352798; 3079812; 30914642; 3100390; 31369031; 31393857; 3166417; 31924474; 3220432; 32269095; 32450896; 33176797; 33361812; 3426502; 3475376; 3569904; 3876282; 3971387; 4042679; 4080829; 4209112; 4309830; 4349512; 4397868; 4634048; 4689339; 479550; 4824507; 5031475; 5358144; 536693; 5367378; 5424503; 5496133; 5527661; 5558564; 5650006; 5698855; 5726144; 5902512; 5948402; 5962396; 6064106; 6067640; 608946; 6184409; 6297853; 6330270; 6334582; 6466874; 6607530; 6696991; 6706951; 6707444; 6831040; 7241566; 7247077; 7439685; 7479744; 7508415; 7607689; 7647459; 7665913; 7673350; 7696204; 7774911; 7792254; 7806216; 7813775; 782223; 7849740; 7886003; 7894156; 7916309; 791749; 7927709; 7949731; 8009369; 8027058; 8040609; 8054974; 8069308; 8088529; 8088814; 8098006; 8248019; 8286607; 8355697; 8391931; 8432998; 8433000; 8482578; 8499946; 8535092; 8601447; 8650184; 8661718; 8703136; 8747923; 8759137; 8783939; 8812411; 8838309; 8875886; 8903730; 8921974; 9133412; 9133672; 9205110; 9321476; 9364439; 9510032; 9548375; 9585436; 9598328; 9685680; 9822646; 9840923; 9918801; |
| Motif | |
| Gene Encoded By | |
| Mass | 60,761 |
| Kinetics | |
| Metal Binding | METAL 192; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 215; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 224; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 377; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 381; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 404; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643 |
| Rhea ID | |
| Cross Reference Brenda |