| IED ID | IndEnz0016000006 |
| Enzyme Type ID | tyrosinase000006 |
| Protein Name |
L-dopachrome tautomerase DCT DT EC 5.3.3.12 L-dopachrome Delta-isomerase Tyrosinase-related protein 2 TRP-2 TRP2 |
| Gene Name | DCT TYRP2 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNALEGFDKADGTLDSQVMSLHNLVHSFLNGTNALPHSAANDPIFVVLHSFTDAIFDEWMKRFNPPADAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTSDQLGYSYAIDLPVSVEETPGWPTTLLVVMGTLVALVGLFVLLAFLQYRRLRKGYTPLMETHLSSKRYTEEA |
| Enzyme Length | 519 |
| Uniprot Accession Number | P40126 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate; Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509; EC=5.3.3.12; Evidence={ECO:0000250|UniProtKB:P29812}; |
| DNA Binding | |
| EC Number | 5.3.3.12 |
| Enzyme Function | FUNCTION: Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA). {ECO:0000269|PubMed:33100333, ECO:0000269|PubMed:8306979}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis; melanin biosynthesis. {ECO:0000269|PubMed:33100333}. |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Glycosylation (6); Metal binding (6); Natural variant (2); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | 3D-structure;Albinism;Alternative splicing;Disease variant;Glycoprotein;Isomerase;Melanin biosynthesis;Membrane;Metal-binding;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc |
| Interact With | P60201-2 |
| Induction | INDUCTION: Induced by blue light (415nm). {ECO:0000269|PubMed:28842328}. |
| Subcellular Location | SUBCELLULAR LOCATION: Melanosome membrane {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}; Single-pass type I membrane protein {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000250|UniProtKB:P29812}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. {ECO:0000250|UniProtKB:P29812}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000250|UniProtKB:P29812}. |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4HX1; |
| Mapped Pubmed ID | 12559959; 15856458; 16857183; 17200659; 17766092; 18206123; 18312627; 18337837; 18674612; 19284502; 19855129; 20221248; 20356546; 20520707; 22898827; 22988252; 23566939; 24709887; 25346513; 27053106; 28095444; 28923927; 31208857; 33959807; 34107850; 35163231; 8048919; |
| Motif | |
| Gene Encoded By | |
| Mass | 59,145 |
| Kinetics | |
| Metal Binding | METAL 189; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 211; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 220; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 369; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 373; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 396; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250 |
| Rhea ID | RHEA:13041 |
| Cross Reference Brenda |