| IED ID | IndEnz0016000012 |
| Enzyme Type ID | tyrosinase000012 |
| Protein Name |
Tyrosinase EC 1.14.18.1 Monophenol monooxygenase |
| Gene Name | T 90C4.150 NCU00776 |
| Organism | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Sordariaceae Neurospora Neurospora crassa Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
| Enzyme Sequence | MSTDIKFAITGVPTPPSSNGAVPLRRELRDLQQNYPEQFNLYLLGLRDFQGLDEAKLDSYYQVAGIHGMPFKPWAGVPSDTDWSQPGSSGFGGYCTHSSILFITWHRPYLALYEQALYASVQAVAQKFPVEGGLRAKYVAAAKDFRAPYFDWASQPPKGTLAFPESLSSRTIQVVDVDGKTKSINNPLHRFTFHPVNPSPGDFSAAWSRYPSTVRYPNRLTGASRDERIAPILANELASLRNNVSLLLLSYKDFDAFSYNRWDPNTNPGDFGSLEDVHNEIHDRTGGNGHMSSLEVSAFDPLFWLHHVNVDRLWSIWQDLNPNSFMTPRPAPYSTFVAQEGESQSKSTPLEPFWDKSAANFWTSEQVKDSITFGYAYPETQKWKYSSVKEYQAAIRKSVTALYGSNVFANFVENVADRTPALKKPQATGEESKSTVSAAAAHAVELSGAKKVAEKVHNVFQHAEEKAQKPVVPVKDTKAESSTAAGMMIGLSIKRPSKLTASPGPIPESLKYLAPDGKYTDWIVNVRAQKHGLGQSFRVIVFLGEFNPDPETWDDEFNCVGRVSVLGRSAETQCGKCRKDNANGLIVSGTVPLTSALLQDIVGGELQSLKPEDVIPHLRANLKWKVALFNGDEYNLEEVPDLKVSVASTEVTIDEEGLPHYSRQYTVYPEITEGKPCGHGPEDHI |
| Enzyme Length | 685 |
| Uniprot Accession Number | P00440 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; |
| DNA Binding | |
| EC Number | 1.14.18.1 |
| Enzyme Function | FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Cross-link (1); Erroneous gene model prediction (1); Frameshift (2); Initiator methionine (1); Metal binding (6); Modified residue (1); Natural variant (9); Propeptide (1); Sequence conflict (2) |
| Keywords | Acetylation;Copper;Direct protein sequencing;Melanin biosynthesis;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Thioether bond |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 2; /note=N-acetylserine; /evidence=ECO:0000269|PubMed:6210696 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 75,886 |
| Kinetics | |
| Metal Binding | METAL 67; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 97; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 106; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 278; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 282; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 307; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19 |
| Rhea ID | RHEA:34287; RHEA:18117 |
| Cross Reference Brenda |