| IED ID | IndEnz0016000015 |
| Enzyme Type ID | tyrosinase000015 |
| Protein Name |
Tyrosinase P EC 1.14.18.- Melanin biosynthesis protein B |
| Gene Name | tyrP melB ATEG_03564 |
| Organism | Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Enzyme Sequence | MGFYRNLVLVAASCTQALGLCPAPRCDSPDIRHEWGELSREDRLSYISAVQCMKDRPPELSVEEVPAVRSRYDDFTAVHINYTLQIHNSGIFLPWHRHFIWLWEKALREECGFTGTLPYWDWVMWPNLAASPLFDGTETSLSGDGEFNATEQPTELNPEPGLTITIPRGAGGGCVRTGPFKDWVINMGPFAFNESYEPALPDHAFDYNPRCLVRSLNDWVIQTYNNQTVVDTLLDSPDIVEFQNIMGGFPNPPIPIGPHAMGHRSLGPDMLDFFASPQDPAFWQHHGMVDRLWTVWQDADEPWRRFALNGSSTTWYKDDTPEVTLQTTVEFGILDEPRPLYELMSPTAGPYCYTYT |
| Enzyme Length | 356 |
| Uniprot Accession Number | Q0CRX0 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Activity is inhibited by the presence of dithiothreitol (DTT). {ECO:0000269|PubMed:27133313}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.14.18.- |
| Enzyme Function | FUNCTION: Tyrosinase; part of the gene cluster that mediates the biosynthesis of Asp-melanin, a pigment that confers resistance against UV light and hampers phagocytosis by soil amoeba (PubMed:28791090, PubMed:27133313, PubMed:29270299). The nonribosomal peptide synthase melA converts 4-hydroxyphenylpyruvate (4-HPPA) to aspulvinone E (PubMed:27133313, PubMed:29270299). The tyrosinase tyrP then performs hydroxylations of both aromatic moieties of aspulvinone E (PubMed:27133313). The product of tyrP is highly unstable, and, due to the high reactivity of methides and ortho-diquinones, the polymeric Asp-melanin forms spontaneously (PubMed:27133313). {ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:28791090, ECO:0000269|PubMed:29270299}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-7. {ECO:0000269|PubMed:27133313}; |
| Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:28791090}. |
| nucleotide Binding | |
| Features | Chain (1); Erroneous gene model prediction (1); Glycosylation (5); Metal binding (5); Signal peptide (1) |
| Keywords | Copper;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Metal-binding;Oxidoreductase;Reference proteome;Signal |
| Interact With | |
| Induction | INDUCTION: Expression is induced during conidiation. {ECO:0000269|PubMed:27133313}. |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:29270299}. Golgi apparatus lumen {ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:29270299}. Note=The oxidizing environment of Golgi or endoplasmic reticulum (ER) is required for tyrP to be active. {ECO:0000269|PubMed:29270299}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:27133313}. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,470 |
| Kinetics | |
| Metal Binding | METAL 87; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 96; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 203; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 263; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 286; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19 |
| Rhea ID | |
| Cross Reference Brenda |