| IED ID | IndEnz0016000019 |
| Enzyme Type ID | tyrosinase000019 |
| Protein Name |
L-dopachrome tautomerase DCT DT EC 5.3.3.12 L-dopachrome Delta-isomerase Tyrosinase-related protein 2 TRP-2 TRP2 |
| Gene Name | DCT TYRP2 |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MSPLGWGLLLGCLGCALPSGARAQFPRVCMTVGSLQAKECCPPLGADPANVCGSREGRGQCAEVQTDTRPWSGPYVLRNQDDRERWPRKFFDRTCRCTGNFAGYNCGNCRFGWTGPKCDQKKPLVVRRDVHSLTPQEREQFLDALDLAKYTLHPDYVITTQHWLGLLGPNGTRPQIANCSIYDFFVWLHYYSVRDTLLGPGRPYKAIDFSHQGPAFVTWHRYHLLWLERDLQRLTGNESFALPYWNFATGRNECDVCTDQLLGAARQDDPTLISQNSRFSSWEIVCDSLNDYNRRVTLCNGTYEGLLKRNQMGRNSEKLPTLKDIQNCLSLKKFDSPPFFQNSTLSFRNALEGFGKADGTLDSQVMNFHNLVHSFLNGTSALPHSAANDPVFVVLHSFTDAIFDEWMKRFNPPVDAWPRELAPIGHNRMYNMVPFFPPVTNEELFLTADQLGYSYAIDLPVEETPDWTTVLSVVTGMLVVLVVLFALLLFLQYRRLRKGYTPLVETQLSNKRYTEEA |
| Enzyme Length | 517 |
| Uniprot Accession Number | Q95119 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate; Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509; EC=5.3.3.12; Evidence={ECO:0000250|UniProtKB:P29812}; |
| DNA Binding | |
| EC Number | 5.3.3.12 |
| Enzyme Function | FUNCTION: Plays a role in melanin biosynthesis. Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA). {ECO:0000250|UniProtKB:P40126}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis; melanin biosynthesis. |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (6); Metal binding (6); Natural variant (2); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Glycoprotein;Isomerase;Melanin biosynthesis;Membrane;Metal-binding;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Melanosome membrane {ECO:0000250|UniProtKB:P40126}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P40126}. Melanosome {ECO:0000250|UniProtKB:P29812}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. {ECO:0000250|UniProtKB:P29812}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000250|UniProtKB:P29812}. |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 58,787 |
| Kinetics | |
| Metal Binding | METAL 189; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 211; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 220; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 369; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 373; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250; METAL 396; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250 |
| Rhea ID | RHEA:13041 |
| Cross Reference Brenda |