| IED ID | IndEnz0016000023 |
| Enzyme Type ID | tyrosinase000023 |
| Protein Name |
Tyrosinase EC 1.14.18.1 Monophenol monooxygenase |
| Gene Name | melO AO090038000061 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MASVEPIKTFEIRQKGPVETKAERKSIRDLNEEELDKLIEAWRWIQDPARTGEDSFFYLAGLHGEPFRGAGYNNSHWWGGYCHHGNILFPTWHRAYLMAVEKALRKACPDVSLPYWDESDDETAKKGIPLIFTQKEYKGKPNPLYSYTFSERIVDRLAKFPDADYSKPQGYKTCRYPYSGLCGQDDIAIAQQHNNFLDANFNQEQITGLLNSNVTSWLNLGQFTDIEGKQVKADTRWKIRQCLLTEEYTVFSNTTSAQRWNDEQFHPLESGGKETEAKATSLAVPLESPHNDMHLAIGGVQIPGFNVDQYAGANGDMGENDTASFDPIFYFHHCFIDYLFWTWQTMHKKTDASQITILPEYPGTNSVDSQGPTPGISGNTWLTLDTPLDPFRENGDKVTSNKLLTLKDLPYTYKAPTSGTGSVFNDVPRLNYPLSPPILRVSGINRASIAGSFALAISQTDHTGKAQVKGIESVLSRWHVQGCANCQTHLSTTAFVPLFELNEDDAKRKHANNELAVHLHTRGNPGGQRVRNVTVGTMR |
| Enzyme Length | 539 |
| Uniprot Accession Number | Q00234 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Activated by acidifying treatment at pH 3.0. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; Evidence={ECO:0000269|PubMed:7893753}; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; Evidence={ECO:0000269|PubMed:7893753}; |
| DNA Binding | |
| EC Number | 1.14.18.1 |
| Enzyme Function | FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Cross-link (1); Metal binding (6) |
| Keywords | Copper;Direct protein sequencing;Melanin biosynthesis;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Thioether bond |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,605 |
| Kinetics | |
| Metal Binding | METAL 63; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 84; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 93; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 290; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 294; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 333; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19 |
| Rhea ID | RHEA:34287; RHEA:18117 |
| Cross Reference Brenda |