| IED ID | IndEnz0016000026 |
| Enzyme Type ID | tyrosinase000026 |
| Protein Name |
Tyrosinase EC 1.14.18.1 Monophenol monooxygenase |
| Gene Name | tyr tyro |
| Organism | Oryzias latipes (Japanese rice fish) (Japanese killifish) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Euteleosteomorpha Neoteleostei Eurypterygia Ctenosquamata Acanthomorphata Euacanthomorphacea Percomorphaceae Ovalentaria Atherinomorphae Beloniformes (medakas needlefish and others) Adrianichthyoidei Adrianichthyidae Oryziinae (medakas) Oryzias Oryzias latipes (Japanese rice fish) (Japanese killifish) |
| Enzyme Sequence | MKSLFLSAVLLQFFETCWSQFPRPCANSEGLRTKECCPVWSGDGSPCGALSGRGFCADVSVSDEPNGPQYPHSGIDDRERWPLAFFNRTCRCAGNYGGFNCGECRFGYWGSNCAEYRESVRRNIMSMSTTEQQKFISYLNLAKNTINPDYVITTGTRAEMGENGESPMFSDINTYDLFVWIHYYVSRDTFLGGPGNVWRDIDFAHESAAFLPWHRVYLLHWEQEIRKITGDFNFTIPYWDWRDAQSCEVCTDNLMGGRNALNPNLISPASVFSSWKVICTQQEEYNNQEALCNATAEGPLLRNPGNHDPNRVPRIPTTADVEFTISLPEYETGSMDRFANNSFRNVLEGFASPETGMAVQGQSTMHNALHVFMNGSMSSVQGSANDPIFLLHHAFIDSIFERWLRTHQPPRSIYPRTNAPIGHNDGYYMVPFLPLYRNGDYLLSNKALGYEYAYLLDPGQRFVQEFLTPYLQQAQQIWQWLLGAGILGALIATIVAAVIVFARRKRRRNQKRKRAPSFGERQPLLQSSSEEGSSSYQTTL |
| Enzyme Length | 540 |
| Uniprot Accession Number | P55025 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; |
| DNA Binding | |
| EC Number | 1.14.18.1 |
| Enzyme Function | FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Glycosylation (5); Metal binding (6); Region (1); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Copper;Glycoprotein;Melanin biosynthesis;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I membrane protein. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 61,254 |
| Kinetics | |
| Metal Binding | METAL 182; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 205; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 214; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 366; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 370; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 393; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19 |
| Rhea ID | RHEA:34287; RHEA:18117 |
| Cross Reference Brenda |