Detail Information for IndEnz0016000035
IED ID IndEnz0016000035
Enzyme Type ID tyrosinase000035
Protein Name Tyrosinase-like protein orsC
EC 1.14.-.-
Orsellinic acid/F9775 biosynthesis cluster protein C
Gene Name orsC AN7912
Organism Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Enzyme Sequence MLAFNPLVTALAALIFLFCQANANPPLMQRLVHEYQWKTKQGLPRQGACTPHNLAVRREWSTLDVETRLEYIEAVKCLARLPSIIDPELAPGARSRFDDFQATHIRHTRTIHATGSFFAWHRHFVYLYEKALREECGYTGYQPYWEWSHWANLPITANPLYDGSNASLSGNGVYIPNRNGTLQLFPIPNPSPDTAIYTPPGTGGGYIYDGPLVDWELHLGPVLYSYDNGQYIPPNPRPDGLGYNPRPLIRDFNNTLLQQGASWDIILNMLVNVTDMHEFHPLFFQGPHLAGHIFISGVDNDIFTSPGDPLFWFHHAQVDRIWTIWQALDLETREYALDGTLTLLNCKNLPFRRILAGELTVCDDSTAQP
Enzyme Length 369
Uniprot Accession Number Q5AUW8
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 1.14.-.-
Enzyme Function FUNCTION: Tyrosinase-like protein; part of the gene cluster that mediates the biosynthesis of orsellinic acid, as well as of the cathepsin K inhibitors F9775 A and F9775 B (PubMed:19666480, PubMed:20174687). The non-reducing polyketide synthase orsA produces orsellinic acid by condensing acetyl-CoA with 3 malonyl-CoA units (PubMed:19666480, PubMed:20174687). Further modifications by the decarboxylase orsB and the tyrosinase-like protein orsC lead to the production of F9775 A and F9775 B (PubMed:20174687). The functions of orsD and orsE remain unclear since only orsB and orsC are required to convert orsellinic acid into F9775 A and F9775 B (PubMed:20174687). {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:20174687}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:20174687}.
nucleotide Binding
Features Chain (1); Glycosylation (4); Metal binding (3); Signal peptide (1)
Keywords Copper;Glycoprotein;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal
Interact With
Induction INDUCTION: Expression is induced by an intimate physical interaction of the fungal mycelia with the bacterium Streptomyces hygroscopicus (PubMed:19666480). Expression is repressed by VeA and MvlA via histone 3 acetylation by the SAGA/ADA complex (PubMed:23892751, PubMed:23841751). {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:23841751, ECO:0000269|PubMed:23892751}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 41,912
Kinetics
Metal Binding METAL 112; /note=Copper A; /evidence=ECO:0000250|UniProtKB:P06845; METAL 121; /note=Copper A; /evidence=ECO:0000250|UniProtKB:P06845; METAL 315; /note=Copper B; /evidence=ECO:0000250|UniProtKB:P06845
Rhea ID
Cross Reference Brenda