| IED ID |
IndEnz0016000036 |
| Enzyme Type ID |
tyrosinase000036 |
| Protein Name |
Orsellinic acid/F9775 biosynthesis cluster protein D
|
| Gene Name |
orsD AN7913 |
| Organism |
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Pezizomycotina
leotiomyceta
Eurotiomycetes
Eurotiomycetidae
Eurotiales (green and blue molds)
Aspergillaceae
Aspergillus
Aspergillus subgen. Nidulantes
Emericella nidulans (Aspergillus nidulans)
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
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| Enzyme Sequence |
MPPVRFGPRKILEYDGRYGVLICHECRYAIQKSALQSHLLRHKIYRADRQQLVAMINELDLLEPDDVLLPPPESPPIDGLPVIAGYRCTAPGCANLCASLKRMKGHWRESHGIADASLARPAKLQTFFRGTKIRYFEVTPTTEDEDDEENESENDEEEGDVDLEEQEDDNGGRQSTTVTTSPGPSAPSVNVDLETLSYFHHFMSATSLTLPCPQDMQSGAQYWKEKAVPQALQQRWLMCGLLALAACHLAAFPDNAAAGQQHRKRAAEFSLEFRTGWRELADTSGEGLREVATEIECLLRCAHWALAESPCDQRIMPEPGVPEHLQSIISTIQSTVPAAAPHEAETSAYATRILRWNTSEAGNSVLAEIRNRLHDLPARMADTFGRPENIQDVLVLLSALAAMGECCDTSFASEEVGPAWWGMATWWTRVPLRFKELVARHYPASLVVVAHWAALMVNRTERCGCWLVKGLAMTILLRIAERLPEDDDGNVQRLVALTIAA |
| Enzyme Length |
501 |
| Uniprot Accession Number |
Q5AUW7 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
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| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Part of the gene cluster that mediates the biosynthesis of orsellinic acid, as well as of the cathepsin K inhibitors F9775 A and F9775 B (PubMed:19666480, PubMed:20174687). The non-reducing polyketide synthase orsA produces orsellinic acid by condensing acetyl-CoA with 3 malonyl-CoA units (PubMed:19666480, PubMed:20174687). Further modifications by the decarboxylase orsB and the tyrosinase-like protein orsC lead to the production of F9775 A and F9775 B (PubMed:20174687). The functions of orsD and orsE remain unclear since only orsB and orsC are required to convert orsellinic acid into F9775 A and F9775 B (PubMed:20174687). {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:20174687}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
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| nucleotide Binding |
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| Features |
Chain (1); Compositional bias (2); Region (1) |
| Keywords |
Reference proteome |
| Interact With |
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| Induction |
INDUCTION: Expression is induced by an intimate physical interaction of the fungal mycelia with the bacterium Streptomyces hygroscopicus (PubMed:19666480). Expression is repressed by VeA and MvlA via histone 3 acetylation by the SAGA/ADA complex (PubMed:23892751, PubMed:23841751). {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:23841751, ECO:0000269|PubMed:23892751}. |
| Subcellular Location |
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| Modified Residue |
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| Post Translational Modification |
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| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
55,729 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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