| IED ID | IndEnz0016000049 |
| Enzyme Type ID | tyrosinase000049 |
| Protein Name |
Ankyrin repeat domain-containing protein 27 VPS9 domain-containing protein VPS9-ankyrin-repeat protein |
| Gene Name | Ankrd27 Varp |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MALYDEDLLKNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPGSVQASCQFESYVLVPTEGHFQTLDGKAVVIEGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLERRETSEEPSAPADPFSLKTIEDVREFLGRHSEKFDKNIASFHRTFRECERKSLRHHIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSAKDELGYCLTSVEAAIEYIRQGSLSTKTPDAEGFGDRLFLKQRMNLLSQMTSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLISGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKASTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAVQNRLKETPLKCALNSKILSIMEAHHLSSDRRPRPSEVPAQSPTRSVDSISQGSSTSSFSSISVSFRQEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDVEDAISTVDLEFCHPLCQCPKCAPAQKLARISANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKKDLSGNTPLICACSAGHHEVAALLLQHGASINACNNKGNTALHEAVMGRHTLVVELLLFYGASVDILNKRQYTAADCAEQDSKIMELLQVVPGCVASLDSVEEADHEGYVTVEIRRKWNPKMYNLPEEPLRRQFCLINPGGRFQERTSRETMGRDRSVPDLAGRSLQEPEKQRVTGKQSDLSDLSRYQTSEEGNKGLPERPVSRQAAPGHRPMVRRHTVNDAAILQVPEVTVHLTTHEASVPQS |
| Enzyme Length | 1048 |
| Uniprot Accession Number | Q3UMR0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560). {ECO:0000250|UniProtKB:Q96NW4, ECO:0000269|PubMed:19403694, ECO:0000269|PubMed:21187289, ECO:0000269|PubMed:26620560}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Chain (1); Compositional bias (2); Domain (1); Frameshift (1); Modified residue (3); Mutagenesis (6); Region (8); Repeat (11); Sequence conflict (6) |
| Keywords | ANK repeat;Alternative splicing;Cell membrane;Cytoplasmic vesicle;Endosome;GTPase activation;Guanine-nucleotide releasing factor;Lysosome;Membrane;Phosphoprotein;Protein transport;Reference proteome;Repeat;Transport |
| Interact With | Q8QZZ8 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Early endosome {ECO:0000250|UniProtKB:Q96NW4}. Late endosome {ECO:0000250|UniProtKB:Q96NW4}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q96NW4}. Lysosome {ECO:0000250|UniProtKB:Q96NW4}. Cell membrane {ECO:0000250|UniProtKB:Q96NW4}. Melanosome {ECO:0000269|PubMed:19403694}. Cytoplasmic vesicle {ECO:0000269|PubMed:19403694}. Note=Colocalizes with VAMP7 in transport vesicles in the shaft of hippocampal neurons. {ECO:0000269|PubMed:19745841}. |
| Modified Residue | MOD_RES 961; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:21183079"; MOD_RES 969; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:Q96NW4"; MOD_RES 1022; /note="Phosphothreonine"; /evidence="ECO:0000250|UniProtKB:Q96NW4" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12466851; 14610273; 17967808; 18477474; 18799693; 21187829; 21267068; 25661869; 27066885; 27626380; |
| Motif | |
| Gene Encoded By | |
| Mass | 116,809 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |