Detail Information for IndEnz0016000050
IED ID IndEnz0016000050
Enzyme Type ID tyrosinase000050
Protein Name +
-larreatricin hydroxylase, chloroplastic
EC 1.14.99.47
Polyphenol oxidase
Gene Name
Organism Larrea tridentata (Creosote bush) (Zygophyllum tridentatum)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Zygophyllales Zygophyllaceae Larreoideae Larrea Larrea tridentata (Creosote bush) (Zygophyllum tridentatum)
Enzyme Sequence MASLSSQSKLLATPYSFPYHTKPSRVSLRRVSCKASNDNKDKPNDQEKTFSIDRRNMLIGLGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCCPPDVKDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVVRAKKARLFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVDRMWYIWKNSLGRKDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPSAADQTEFAGSFVNVPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIEFVRDEED
Enzyme Length 584
Uniprot Accession Number Q6UIL3
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(+)-larreatricin + AH2 + O2 = (+)-3'-hydroxylarreatricin + A + H2O; Xref=Rhea:RHEA:34259, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499, ChEBI:CHEBI:67153, ChEBI:CHEBI:67154; EC=1.14.99.47; Evidence={ECO:0000269|PubMed:12960376};
DNA Binding
EC Number 1.14.99.47
Enzyme Function FUNCTION: Enantio-specific polyphenol oxidase involved in aromatic ring hydroxylation. Involved in the biosynthesis of the creosote bush 8-8' linked lignans. Has a strong preference for the 3' position of (+)-larreatricin. {ECO:0000269|PubMed:12960376}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Cross-link (1); Disulfide bond (2); Metal binding (6); Propeptide (1); Transit peptide (2)
Keywords Chloroplast;Copper;Disulfide bond;Metal-binding;Oxidoreductase;Plastid;Thioether bond;Thylakoid;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen {ECO:0000305|PubMed:12960376}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 66,352
Kinetics
Metal Binding METAL 167; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 188; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 197; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 319; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 323; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 353; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19
Rhea ID RHEA:34259
Cross Reference Brenda 1.14.18.1;1.14.99.47;