| IED ID | IndEnz0016000052 |
| Enzyme Type ID | tyrosinase000052 |
| Protein Name |
Hypoxia-inducible factor 1 Hypoxia-induced factor 1 |
| Gene Name | hif-1 F38A6.3 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MEDNRKRNMERRRETSRHAARDRRSKESDIFDDLKMCVPIVEEGTVTHLDRIALLRVAATICRLRKTAGNVLENNLDNEITNEVWTEDTIAECLDGFVMIVDSDSSILYVTESVAMYLGLTQTDLTGRALRDFLHPSDYDEFDKQSKMLHKPRGEDTDTTGINMVLRMKTVISPRGRCLNLKSALYKSVSFLVHSKVSTGGHVSFMQGITIPAGQGTTNANASAMTKYTESPMGAFTTRHTCDMRITFVSDKFNYILKSELKTLMGTSFYELVHPADMMIVSKSMKELFAKGHIRTPYYRLIAANDTLAWIQTEATTITHTTKGQKGQYVICVHYVLGIQGAEESLVVCTDSMPAGMQVDIKKEVDDTRDYIGRQPEIVECVDFTPLIEPEDPFDTVIEPVVGGEEPVKQADMGARKNSYDDVLQWLFRDQPSSPPPARYRSADRFRTTEPSNFGSALASPDFMDSSSRTSRPKTSYGRRAQSQGSRTTGSSSTSASATLPHSANYSPLAEGISQCGLNSPPSIKSGQVVYGDARSMGRSCDPSDSSRRFSALSPSDTLNVSSTRGINPVIGSNDVFSTMPFADSIAIAERIDSSPTLTSGEPILCDDLQWEEPDLSCLAPFVDTYDMMQMDEGLPPELQALYDLPDFTPAVPQAPAARPVHIDRSPPAKRMHQSGPSDLDFMYTQHYQPFQQDETYWQGQQQQNEQQPSSYSPFPMLS |
| Enzyme Length | 719 |
| Uniprot Accession Number | G5EGD2 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: A transcription factor which is a key regulator in various cellular processes; including environment stress resistance (oxygen levels, hydrogen sulfide and cyanide levels and heat), negative regulation of cell apoptosis in ASJ neurons by inhibition of cep-1 via transcriptional activation of tyr-2, resistance/susceptibility to pathogenic bacteria, lifespan and brood size (PubMed:11427734, PubMed:12006646, PubMed:12686697, PubMed:15781453, PubMed:18477695, PubMed:19461873, PubMed:21093262, PubMed:19889840, PubMed:20520707, PubMed:20400959). Involved in mediating susceptibility to enteropathogenic E.coli (PubMed:16091039). Increased levels of hif-1 activity confer resistance to P.aeruginosa-mediated death but also confer susceptibility to S.aureus infection (PubMed:20865124, PubMed:22792069). Required for aha-1 nuclear localization (PubMed:11427734). Following hypoxic stress, up-regulates serotonin levels through activation of tph-1 expression (PubMed:20400959). Role in life span extension is dependent of temperature (PubMed:21241450). Not required for survival in anoxic conditions (PubMed:11427734). Involved in iron homeostasis by repressing transcription of ferritin ftn-1 and ftn-2, and divalent metal transporter smf-3 (PubMed:22396654, PubMed:22194696). May be involved in manganese homeostasis by repressing divalent metal transporter smf-3 (PubMed:22194696). {ECO:0000269|PubMed:11427734, ECO:0000269|PubMed:12006646, ECO:0000269|PubMed:12686697, ECO:0000269|PubMed:15781453, ECO:0000269|PubMed:16091039, ECO:0000269|PubMed:18477695, ECO:0000269|PubMed:19461873, ECO:0000269|PubMed:19889840, ECO:0000269|PubMed:20400959, ECO:0000269|PubMed:20520707, ECO:0000269|PubMed:20865124, ECO:0000269|PubMed:21093262, ECO:0000269|PubMed:21241450, ECO:0000269|PubMed:22194696, ECO:0000269|PubMed:22396654, ECO:0000269|PubMed:22792069}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (5); Chain (1); Compositional bias (1); Domain (4); Modified residue (1); Mutagenesis (1); Region (5) |
| Keywords | Alternative splicing;Apoptosis;DNA-binding;Hydroxylation;Nucleus;Reference proteome;Repeat;Repressor;Stress response;Transcription;Transcription regulation;Two-component regulatory system |
| Interact With | O02219 |
| Induction | INDUCTION: By heat acclimation, hypoxia and addition of 50 ppm hydrogen sulfide (PubMed:11427734, PubMed:12686697, PubMed:19889840). By low iron levels (PubMed:22194696). {ECO:0000269|PubMed:11427734, ECO:0000269|PubMed:12686697, ECO:0000269|PubMed:19889840, ECO:0000269|PubMed:22194696}. |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:19889840}. |
| Modified Residue | MOD_RES 621; /note=4-hydroxyproline; by egl-9; /evidence=ECO:0000269|PubMed:11595184 |
| Post Translational Modification | PTM: Hydroxylation on Pro-621 by egl-9 during normoxia conditions is required for vhl-1-mediated proteasomal degradation. {ECO:0000269|PubMed:11595184}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10615043; 10973497; 12050673; 12679813; 12824472; 15184665; 16040138; 16980385; 17164286; 17873906; 18524954; 18587389; 19050081; 19343510; 19372390; 19576771; 19632181; 19633713; 19737748; 20011506; 20439774; 20599952; 21110867; 21177967; 21212236; 21840852; 21980473; 22128035; 22286215; 22347378; 22405203; 22560298; 22921415; 23419779; 23516373; 23533643; 23566034; 23791784; 23800452; 23825972; 24107417; 25487147; 25510338; 25762526; 26586189; 26677221; 26780296; 26790951; 27420916; 27566309; 27618966; 28179109; 29949401; 30010540; 30773781; 31371406; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,934 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |