| IED ID |
IndEnz0016000076 |
| Enzyme Type ID |
tyrosinase000076 |
| Protein Name |
Microphthalmia-associated transcription factor
|
| Gene Name |
Mitf |
| Organism |
Rattus norvegicus (Rat) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Glires (Rodents and rabbits)
Rodentia
Myomorpha (mice and others)
Muroidea
Muridae
Murinae
Rattus
Rattus norvegicus (Rat)
|
| Enzyme Sequence |
MQSESGIVADFEVGEEFHEEPKTYYELKSQPLKSSSSAEHSGASKPPLSSSTMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVAVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRHQVKQYLSTTLANKHAGQVLSPPCPNQPGDHAMPPVPGSSAPNSPMAMLTLNSNCEKEAFYKFEEQSRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYSNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKDLENRQKKLEHANRHLLLRVQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQELVQHQADLTCTTTLDLTDGTISFTNNLGTMPESSPAYSIPRKMASNLEDILMDDALSPVGVTDPLLSSVSPGASKTSSRRSSMSAEETEHAC |
| Enzyme Length |
526 |
| Uniprot Accession Number |
O88368 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. {ECO:0000250|UniProtKB:O75030}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
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| nucleotide Binding |
|
| Features |
Chain (1); Coiled coil (1); Compositional bias (2); Cross-link (2); Domain (1); Modified residue (5); Region (3) |
| Keywords |
Activator;Coiled coil;Cytoplasm;DNA-binding;Developmental protein;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation |
| Interact With |
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| Induction |
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| Subcellular Location |
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75030}. Cytoplasm {ECO:0000250|UniProtKB:O75030}. Note=Found exclusively in the nucleus upon phosphorylation. {ECO:0000250|UniProtKB:O75030}. |
| Modified Residue |
MOD_RES 180; /note=Phosphoserine; by MAPK; /evidence=ECO:0000250|UniProtKB:O75030; MOD_RES 405; /note=Phosphoserine; by GSK3; /evidence=ECO:0000250|UniProtKB:O75030; MOD_RES 414; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:O75030; MOD_RES 491; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:O75030; MOD_RES 516; /note=Phosphoserine; by RPS6KA1; /evidence=ECO:0000250|UniProtKB:O75030 |
| Post Translational Modification |
PTM: Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (By similarity). Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (By similarity). Phosphorylated in response to blue light (415nm) (By similarity). {ECO:0000250|UniProtKB:O75030}.; PTM: Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation. {ECO:0000250|UniProtKB:O75030}. |
| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
19201870;
19524539;
21893222;
22576626;
26388265;
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| Motif |
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| Gene Encoded By |
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| Mass |
58,585 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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