| IED ID | IndEnz0016000080 |
| Enzyme Type ID | tyrosinase000080 |
| Protein Name |
Probable FAD-dependent monooxygenase kojA EC 1.14.13.- Kojic acid biosynthesis protein A |
| Gene Name | kojA AO090113000136 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MRVATQLRVGIVGGGWNGCHLALELKKQGHRVSLFEQKPDIFQGVSGNFGIRLHKGPHYPRSKATRDSCREALVKFCETYPELVVHHESAIYAHGEADALGNPSKVSDEAFRDVCYESPECTAVDPKANGFQGLISAYNLDEPSVAIGDRLRNTFKEKLGRAGIYVHLNATVDRIIHTEDTNRIQTGDGQYVFDVVINATGYTSLLPQNIADALPVDIGITYQTCIALVYEDQQPQEKPLSFIVMDGWFPCVMPAIDTNEPLQKKYILTHGSYTILGSFDRHEEGQELLDSLDEEAIAARIKPHCEREITRFWPGFLDRFQYRGWKGSVLAKLKTTSEFRSSLTFEKDGVIHIFPGKVSNVVTAAEEVVPLINDIARRRHGVVREWNGVRFTVSSAFHTHSKEIGDKPGLGEHHTSNLQTYVSLVTAN |
| Enzyme Length | 428 |
| Uniprot Accession Number | Q2U5I0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.14.13.- |
| Enzyme Function | FUNCTION: Probable FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of 5-hydroxy-2-hydroxymethyl-1,4-pyrone, also know as kojic acid, a by-product in the fermentation process of malting rice that acts as a chelation agent (PubMed:20849972). Glucose might be converted to kojic acid by a combination of dehydrogenase and dehydratase reactions involving kojA and probably additional enzymes (PubMed:20849972). {ECO:0000269|PubMed:20849972}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 52..60; /note=FAD; /evidence=ECO:0000250|UniProtKB:P20586; NP_BIND 328..329; /note=FAD; /evidence=ECO:0000250|UniProtKB:P20586 |
| Features | Chain (1); Nucleotide binding (2); Region (1); Site (1) |
| Keywords | FAD;Flavoprotein;Monooxygenase;Oxidoreductase;Reference proteome |
| Interact With | |
| Induction | INDUCTION: Expression is controlled by the kojic acid gene cluster transcription factor kojR (PubMed:21514215). Expression is also positively regulated by the secondary metabolism general regulator laeA (PubMed:21897021). Finally, nitrogen deficiency also positively regulates expression (PubMed:26657710). {ECO:0000269|PubMed:21514215, ECO:0000269|PubMed:21897021, ECO:0000269|PubMed:26657710}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,791 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |