Detail Information for IndEnz0016000082
IED ID IndEnz0016000082
Enzyme Type ID tyrosinase000082
Protein Name Probable efflux pump kojT
Kojic acid transporter
Gene Name kojT AO090113000138
Organism Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Enzyme Sequence MQSFRQYRRMRRDLQESIKLHGPYAAAGDRHVQPTDDILEDADDARLEKGIHSMNGHGQSPYSTPGIVLHDGQRVTVPGVNLRRASEICERVNTKTLFIVGFDGPDDQLNPKNWSIGRKWATLGIVGTTGMLVGWASSIDSTVIKQGQEAFGVSEVAESLATALFLFAFGFGSLVAAPFSETVGRNPVYIATLSILMIFTMASGLAPNFGAQLAFRFLAGLFGCTPMTTFGGSMADIFDPMDRTYAFPVCCTLSFLGPFLAPMVGAFIGQSTHISWRWTEWCTLIMAALVTGAIFLFVPETYGPVLLQWKAKQLREITGDPRFMAEIELRQTSLVTRLMHSCSRPFHLFFREIMVALFTMYLVVVYIVLFGFLTGYEFIFGRTYGFTQGSVGLTFIGMNIGFLIAFAMVPHIYFSYKKRLQNAIENGHNGLPPEERLWFAMYGAPWLPISLFWMGWTSYPSISYWSPLVASVAFGFSVQGIFISTYQYLIDTYELFAASALVSATFFRYIAAGAMVIVSIPMYGNLGVHWSLTLLGCISVLMTPVPYIFYKYGHVIRQRNKKTP
Enzyme Length 564
Uniprot Accession Number Q2U5H8
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Probable efflux pump; part of the gene cluster that mediates the biosynthesis of 5-hydroxy-2-hydroxymethyl-1,4-pyrone, also know as kojic acid, a by-product in the fermentation process of malting rice that acts as a chelation agent (PubMed:20849972). Glucose might be converted to kojic acid by a combination of dehydrogenase and dehydratase reactions involving kojA and probably additional enzymes (PubMed:20849972). {ECO:0000269|PubMed:20849972}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Glycosylation (1); Transmembrane (12)
Keywords Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport
Interact With
Induction INDUCTION: Expression is controlled by the kojic acid gene cluster transcription factor kojR (PubMed:21514215). Expression is also positively regulated by the secondary metabolism general regulator laeA (PubMed:21897021). Finally, nitrogen deficiency also positively regulates expression (PubMed:26657710). {ECO:0000269|PubMed:21514215, ECO:0000269|PubMed:21897021, ECO:0000269|PubMed:26657710}.
Subcellular Location SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 62,915
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda