IED ID | IndEnz0016000083 |
Enzyme Type ID | tyrosinase000083 |
Protein Name |
Aspulvinone E synthetase melA EC 2.3.1.- Nonribosomal peptide synthase melA |
Gene Name | melA ATEG_03563 |
Organism | Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
Enzyme Sequence | MQPSLIPSLLETAAARNGDGRVILYSQGNREDPRSITYRDLLETASKASVAVHNHQNYTPGAAVLLHFNNHLDNIVWFWAVLLAGCIPAITPAFSNNPVQRVANLEHLSSTLITDWCLTSQALLVEFAGQDAIEPVSVETLGWEKASPDSNTASVKAKPTDTALLLFTSGSTGKSKAVCLSHFQIVSAIAGKLSVVPLPEQSSFLNWVGLDHVAAIIEIHLQALYADLDQVHVPGSDVISDPIWFLDLMATHRVSRTFAPNFFLARIRDALVQNARSASPRQWDLSGLRYVASGGEANTTKTCDELSQLLKSFGAPLNVIVPGFGMTETCAGAIFNTNCPDYDKKHGLEYTSVGSCMPGIFMRVTNQQGDPLPPGEMGSLELAGPVVFRQYLNNPAATQESFTMDGWFKTGDCGTLDENGYLVLGGRAKETIIINGVKYSPHEIETAVEEHNIKGLSRSFTCCFSSLSPGAETEEIVLVYLPTYAPEDIPARAATADAISKVVLMSTGSRPHIIPLEQALLPKSTLGKLSRSKIKAAYERGEYRTHDSINRSLIARHRQATRASPKNDFEKGLLEIFLRSFKISEDEFDVQTPIFDVGIDSIELINLKRDIEQHLGFADATIPIIILLENTTVRELAAALDNLYRPKEYNPVVTLQAHGDKNPLWLVHPGAGEVLIFINLAKFITDRPVYALRARGFDEGEKPFDSIEDAVTSYYNGVKSKQPHGPYALAGYCYGSMLAFEVAKKLEENGDEVRFVGSFNLPPHIKMRMRELDWKECLLHLAYFLDLITQKRSRELAVELDGLDQDTILQAIIDEADKERYAQLSLSRPFLSRWADVAYELHRIAGDYDPDGRVASMDVFFSIPLAIAAASKSEWRNVHLSQWDDFTRSHVRFHDVPGEHYSMIGPEHVFAFQKILRSALAERGM |
Enzyme Length | 925 |
Uniprot Accession Number | Q0CRX1 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 3-(4-hydroxyphenyl)pyruvate = aspulvinone E + H2O; Xref=Rhea:RHEA:63824, ChEBI:CHEBI:15377, ChEBI:CHEBI:36242, ChEBI:CHEBI:58240; Evidence={ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:29270299, ECO:0000269|PubMed:29305695};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63825; Evidence={ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:29270299, ECO:0000269|PubMed:29305695}; |
DNA Binding | |
EC Number | 2.3.1.- |
Enzyme Function | FUNCTION: Nonribosomal peptide synthase; part of the gene cluster that mediates the biosynthesis of Asp-melanin, a pigment that confers resistance against UV light and hampers phagocytosis by soil amoeba (PubMed:29305695, PubMed:23841722, PubMed:27133313, PubMed:29270299). The nonribosomal peptide synthase melA converts 4-hydroxyphenylpyruvate (4-HPPA) to aspulvinone E (PubMed:29305695, PubMed:27133313, PubMed:29270299). The tyrosinase tyrP then performs hydroxylations of both aromatic moieties of aspulvinone E (PubMed:27133313, PubMed:29305695). The product of tyrP is highly unstable, and, due to the high reactivity of methides and ortho-diquinones, the polymeric Asp-melanin forms spontaneously (PubMed:27133313). {ECO:0000269|PubMed:23841722, ECO:0000269|PubMed:27133313, ECO:0000269|PubMed:29270299, ECO:0000269|PubMed:29305695}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Domain (1); Erroneous gene model prediction (1); Modified residue (1); Region (2) |
Keywords | Cytoplasm;Phosphopantetheine;Phosphoprotein;Reference proteome;Transferase |
Interact With | |
Induction | INDUCTION: Expression is induced during conidiation. {ECO:0000269|PubMed:27133313}. |
Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29270299}. |
Modified Residue | MOD_RES 601; /note=O-(pantetheine 4'-phosphoryl)serine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00258 |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 102,373 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:63824; RHEA:63825 |
Cross Reference Brenda |