Detail Information for IndEnz0016000092
IED ID IndEnz0016000092
Enzyme Type ID tyrosinase000092
Protein Name Phenoloxidase 3
EC 1.14.18.1
Tyrosinase 3
Fragment
Gene Name PPO3
Organism Sarcophaga argyrostoma (Flesh fly) (Liopygia argyrostoma)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Calyptratae Oestroidea Sarcophagidae (flesh flies) Sarcophaginae Sarcophaga Liopygia Sarcophaga argyrostoma (Flesh fly) (Liopygia argyrostoma)
Enzyme Sequence HWHLVYPIEAPDRSIVDKDRRGELFYYMHQQIIARYNAERYNAERLSNHMARVQPFNNLDEPIAEGYFPKM
Enzyme Length 71
Uniprot Accession Number C0HJM0
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; Evidence={ECO:0000269|Ref.1}; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; Evidence={ECO:0000269|Ref.1};
DNA Binding
EC Number 1.14.18.1
Enzyme Function FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone (By similarity). {ECO:0000250|UniProtKB:O44249}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Metal binding (2); Non-terminal residue (2)
Keywords Copper;Direct protein sequencing;Melanin biosynthesis;Metal-binding;Monooxygenase;Oxidoreductase;Secreted;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:O44249}.
Modified Residue
Post Translational Modification PTM: Upon activation, a trypsin type protease cleaves prophenol oxidase to yield the active enzyme. {ECO:0000250|UniProtKB:Q9W1V6}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 8,623
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.159 mM for L-dopa {ECO:0000269|Ref.1}; Vmax=151.3 umol/min/ug enzyme with L-dopa as substrate {ECO:0000269|Ref.1}; Note=Enzymes were activated using methanol. {ECO:0000269|Ref.1};
Metal Binding METAL 3; /note=Copper A; /evidence=ECO:0000250|UniProtKB:O44249; METAL 29; /note=Copper A; /evidence=ECO:0000250|UniProtKB:O44249
Rhea ID RHEA:34287; RHEA:18117
Cross Reference Brenda