| IED ID | IndEnz0016000120 |
| Enzyme Type ID | tyrosinase000120 |
| Protein Name |
Orsellinic acid synthase OAS EC 2.3.1.- Non-reducing polyketide synthase orsA Orsellinic acid/F9775 biosynthesis cluster protein A |
| Gene Name | orsA AN7909 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MAPNHVLFFPQERVTFDAVHDLNVRSKSRRRLQSLLAAASNVVQHWTASLDGLERADIGSFEDLVELAERQTTQTRGSIVADLVLLTTVQIGQLLVLAEDDPAILSGHAGARAIPMGFGAGLVAAGVAAAATSADGIVNLGLEAVSVAFRLGVELQRRGKDIEDSNGPWAQVISSATTIADLEQALDRINASLRPINQAYIGEVMTESTVVFGPPSTLDALAKRPELAHATITSPASALAQVPLHGAHLPPISATMIAASSSQQATELWKLAVEEVANKPIDVHQAVTALIHDLHRANITDIVLTAIGASTETSGIQSLLEKNGLAVELGQLSPTPRPYGNDLDSIPADAIAVVGMSGRFPNSDTLDEFWRLLETATTTHQVIPESRFNVDDFYDPTRAKHNALLARYGCFLKNPGDFDHRLFNISPREAMQMDPVQRMLLMTTYEALEMAGYSPPTPAAPGDSEQAPPRIATYFGQTIDDWKSINDQQGIDTHYLPGVNRGFAPGRLSHFFQWAGGFYSIDTGCSSSATALCLARDALTAGKYDAAVVGGGTLLTAPEWFAGLSQGGFLSPTGACKTYSDSADGYCRGEGVGVVILKRLADAVRSKDNVIAVIAGASRNCNAGAGSITYPGEKAQGALYRRVMRQAAVRPEQVDVVEMHGTGTQAGDRVETHAVQSVFAPSNGNQREKPLIVGALKANIGHSEAAAGIISLMKAILILQHDKIPAQPNQPIKMNPYLEPLIGKQIQLANGQSWTRNGAEPRYIFVNNFDAAGGNVSMLLQDPPAFALPAPASGPGLRTHHVVVTSGRTATAHEANRKRLHAYLSAHPDTNLADLAYTTTARRIHNVHREAYVASSTSDLVRQLEKPLADKVESAPPPAVVFTFTGQGAQSLGMGGALYSTSPTFRRLLDSLQSICEVQGLPTKFLNAIRGSGAEGATVTEVDMQVATVALEIALARYWRSLGIRPTVLIGHSLGEYAALCVAGVLSASDALALAFRRATLIFTRCPPSEAAMLAVGLPMRTVQYRIRDSAATTGCEVCCVNGPSSTVVGGPVAAIQALDEYLKSDGKVSTTRLRVQHAFHTRQMDVLLDELEASAAQVPFHAPTLPVASTVLGRIVRPGEQGVFDANYLRRHTREPVAFLDAVRACETEGLIPDRSFAVEIGPHPICISLMATCLQSAKINAWPSLRRGGDDWQSVSSTLAAAHSAQLPVAWSEFHKDHLDTVRLISDLPTYAFDLKTFWHSYKTPAAAVSAASATPSTTGLSRLASTTLHAVEKLQREEGKILGTFTVDLSDPKLAKAICGHVVDESAICPASIFIDMAYTAAVFLEQENGAGAALNTYELSSLEMHSPLVLREDIEVLPQVWVEAVLDIKSNAVSVHFKGQTSKGAVGYGSATMRLGQPDSAVRRDWSRIQSLVRARVQTLNRSVRPREVHAMDTALFYKVFSEIVDYSAPYHAVQEAVIAADFHDAAVTLQLTPTADLGTFTSSPFAVDALVHVAGFLLNADVRRPKNEVHIANHIGSLRIVGDLSSPGPYHVYATIREQDQKAGTSLCDVYTTDSQDRLVAVCSDICFKKLERDFFALLTGATRGRSTKPVAAAPAKSMAKRARQLAPSPSPSSSSGSNTPMSRSPTPSSVSDMVDLGTELLQAVAEQTGVSVAEMKSSPGTTFTEFGVDSQMAISILANFQRTTAVELPAAFFTNFPTPADAEAELGGSALDDLEEDITKPTPSPEQTQARKQGPAPSQHLLSLVAQALGLEASDLTPSTTFDSVGMDSMLSIKITAAFHAKTGIELPAAFFSANPTVGAAQEALDDDAEEESAPAQTSTNPAKETTIDSSRQHKLDAAVSRASYIHLKALPKGRRIYALESPFLEQPELFDLSIEEMATIFLRTIRRIQPHGPYLIGGWSAGSMYAYEVAHRLTREGETIQALIILDMRAPSLIPTSIVTTDFVDKLGTFEGINRARDLPEDLSVKERAHLMATCRALSRYDAPAFPSDRQPKQVAVVWALLGLDNRPDAPIASMGRPGLDIGKSMYEMNLDEFERYFNSWFYGRRQQFGTNGWEDLLGDHIAVYTVNGDHFSMMCPPYASEVGDIVIETVTRAVE |
| Enzyme Length | 2103 |
| Uniprot Accession Number | Q5AUX1 |
| Absorption | |
| Active Site | ACT_SITE 525; /note=For beta-ketoacyl synthase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10022; ACT_SITE 973; /note=For acyl/malonyl transferase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + 2 H(+) + 3 malonyl-CoA = 3 CO2 + 4 CoA + orsellinate; Xref=Rhea:RHEA:62972, ChEBI:CHEBI:15378, ChEBI:CHEBI:16162, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57384; Evidence={ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:20174687};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62973; Evidence={ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:20174687}; |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of orsellinic acid, as well as of the cathepsin K inhibitors F9775 A and F9775 B (PubMed:19666480, PubMed:20174687). The non-reducing polyketide synthase orsA produces orsellinic acid by condensing acetyl-CoA with 3 malonyl-CoA units (PubMed:19666480, PubMed:20174687). Further modifications by the decarboxylase orsB and the tyrosinase-like protein orsC lead to the production of F9775 A and F9775 B (PubMed:20174687). The functions of orsD and orsE remain unclear since only orsB and orsC are required to convert orsellinic acid into F9775 A and F9775 B (PubMed:20174687). {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:20174687}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:20174687}. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (2); Modified residue (2); Region (8) |
| Keywords | Multifunctional enzyme;Phosphopantetheine;Phosphoprotein;Reference proteome;Repeat;Transferase |
| Interact With | |
| Induction | INDUCTION: Expression is induced by an intimate physical interaction of the fungal mycelia with the bacterium Streptomyces hygroscopicus (PubMed:19666480). Expression is repressed by VeA and MvlA via histone 3 acetylation by the SAGA/ADA complex (PubMed:23892751, PubMed:23841751). {ECO:0000269|PubMed:19666480, ECO:0000269|PubMed:23841751, ECO:0000269|PubMed:23892751}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 1676; /note=O-(pantetheine 4'-phosphoryl)serine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00258; MOD_RES 1775; /note=O-(pantetheine 4'-phosphoryl)serine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00258 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 225,849 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:62972; RHEA:62973 |
| Cross Reference Brenda |