| IED ID | IndEnz0016000159 |
| Enzyme Type ID | tyrosinase000159 |
| Protein Name |
Non-reducing polyketide synthase terA EC 2.3.1.- Terrein biosynthesis cluster protein terA |
| Gene Name | terA ATEG_00145 |
| Organism | Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Enzyme Sequence | MAGSLKLYVFGDEAGDFAGPLQKLCEQRKEVLFLHFLDELNKVLRDEVRRQPRDVRVQIPEFTDVLDLVRHYRDSGSRNQILETTLTLAFRIGVKVHGAAQRLSKHGDAAVAQSWTTLIIGAQKEASIAALAEFNESKGIPHANQAYISANSKDVVSISGPPETVTSLLQHSEYLQKFRTISLDIFAPFHAPHLYNDDDVAEVLQPFSGKNEKRKLFIPVISGLGTLYSPDMDVETLMADVVRDILIRPLLFENVMKTVTETTTASSHQNCQIFSVGPSQAINSLASTLRADTSLNVTTEGPIHTGSPDYEPLNGAQKIAIVGMAGRFPNSDDLESFWSTLQQGLDLHRRVPPDRFDIDAHYDPTGKKLNSTHTPYGCFIEKPGLFDPRFFNMSQREAYQTDPMGRLALVTAYEALEMSGFVPNRTPSSMLDRIGTFYGQTSDDWRTLNAAEKIDMYYIPGTIRAFATGRINYHFKFKGPSYNVDTACSSSFAAIQLACTSLLAKECDTALAGGLNVMTTPDLFAGLSRAHFLSKTGSCKTFDDGADGFCRGDGVGTVVLKRLEDAEADNDPILAVVLGTATNHSSEAVSITRPHGPAQEALYRKILKHTGVDPVDVSYVEMHGTGTQAGDGTEMKSITNVFAPRDKGRRQPDQLIHLGALKANIGHGEASAGVASLIKTVIMMQKNAIPPHVGIKTTMNKTFPHDLNERGVRIAFKETPWVRPDGGKRRAYLNNFGASGGNTGLLLEDRPAPVSTRASDPRTSFVVSLSAKSAYSLDQNINRLATYLEGNPDTSLPALSYTTTARRVHYPHRVSYAVRSIPETIKSLRSAQSKAIKPDPASSGKIAFLFTGQGSHYTALGKQLFEDCQTFRNDLVEFNRIGQKQGFPTFLPLIDGSEDVASLSPVALQLGQSCIQIALARLWKSWGITPSSVLGHSLGEYAALNVAGVLSASDTIYLVGRRAQLLEELCTPGSHKMLAIAASVSSVKEILGDKDIEVACINGPNETVISGLAEQMESYSKTLKATDVKCSLLSTAYAFHSAQIQVIVEQYRKVASSVHFGPPNVPVISPLLGDVVTDGNVFGPDYLCRQAREAVNFMGALKAAESKGVVDNNVIWLEIGPAPVCSAFVKSSLGSKALTLPSLRKQEDVWKTLSGTLSNLYSKGLTIEWEEVHREYEASHTVLALPSYCFEEKNYWLDYHNNWCLTKGQKLVESAAPKRRGRHLLTPSVQKVIKEDFGQTKITVVAESDLSDPDLNHAVTGHLVNGSALCPAGVYAESALTLAGYIYHRVKKTEDIGMDVRALEIVKPLIAKGRDQKEKQVFRITATADQPLKLVKISYNSVSADGSLGVLHATCHVEYGDIKTWRAEWSRLAYLVRSRIDVMNDGVKGRQYQKLDRKAAYEGFSGFVEYDKQYHGMKEVIMDQKNLEATSILEFQPSNDYGEFEIDPRFIDNISHLSGFILNGSGATDTRKQVFVSHGWDHLQIAEPLSSSKSYSNYVKMHEIEKATMAGDVYIFDGEEMVALVGGVKFKAIPRAVINQLLPPVKGSKALEKSAPRQNPKATATKTTQKPQAPVPVPQKQNKAIIDDFLALLCEELGVELSELQDDTAFADIGLDSLMSLSITGRMREELDLEAIPSSVFTDYPTVGQVKELILKLAGSSSDENTTDTPDEEEDPATADADNTEMIRENPLESVSPNVSSSEAMDGFLAIVCEEVGVDLEELLEVQNFAGAGVDSLMSLTITGRAREDLDMDIPSSFFVDYPTVDQARLAIASLMGGGDQTGATTPYSGSDDAKSSTSSLTAGSVLTPDDDMDAQDISQLTRPATSIVLQGSLKTASKTLFLLPDGSGSATSYAALPRVSPDTCVVALNCPFMKTPSEYTTGIEGVAALYLSEIRRRQPEGPYVLGGWSAGGILAYAVACQLIEMGEEIEDLFLIDSPCPINLQPLPSELLHFIDSLGLLGAQGSAPKWLIPHFEASIKNLTAFVPHAMDPDEAPRTHIIWARDGLVSESDEKQFPRSDAEAKSVKFLLDGRQNLGTYGWEKLIGSENISVDFIEGNHFTMMREPKVNQLPSILDRLIGA |
| Enzyme Length | 2081 |
| Uniprot Accession Number | Q0D1N9 |
| Absorption | |
| Active Site | ACT_SITE 488; /evidence=ECO:0000255|PROSITE-ProRule:PRU10022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + 2 H(+) + 3 malonyl-CoA = 3 CO2 + 4 CoA + orsellinate; Xref=Rhea:RHEA:62972, ChEBI:CHEBI:15378, ChEBI:CHEBI:16162, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57384; Evidence={ECO:0000269|PubMed:24816227};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62973; Evidence={ECO:0000269|PubMed:24816227}; |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of terrein, a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities (PubMed:24816227, PubMed:26173180). The first step in the pathway is performed by the polyketide synthase terA that produces 4-hydroxy-6-methylpyranon (4-HMP), orsellinic acid (OA), and 2,3-dehydro-6-hydroxymellein (2,3-dehydro-6-HM) by condensing acetyl-CoA with two, three, or four malonyl-CoA units, respectively (PubMed:24816227). 4-HMP and OA are not pathway intermediates, but are rather shunt or side products (PubMed:24816227). 2,3-dehydro-6-HM is further converted to 6-hydroxymellein (6-HM) by the 6-hydroxymellein synthase terB (PubMed:24816227). The monooxygenases terC and terD, the multicopper oxidase terE and the Kelch-like protein terF are then involved in the transformation of 6-HM to terrein (PubMed:24816227). Even if they are co-regulated with the other terrein cluster genes, terH and terI seem to be dispensable for terrein production; whereas one or both of the 2 transporters terG and terJ are probably required for efficient secretion of metabolites (PubMed:24816227). {ECO:0000269|PubMed:24816227, ECO:0000269|PubMed:26173180}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:24816227, ECO:0000269|PubMed:26173180}. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (3); Domain (2); Modified residue (2); Region (8) |
| Keywords | Multifunctional enzyme;Phosphopantetheine;Phosphoprotein;Reference proteome;Repeat;Transferase |
| Interact With | |
| Induction | INDUCTION: Expression is induced by methionine (PubMed:26173180). Nitrogen starvation induces expression of terA and promotes terrein production during fruit infection, via regulation by areA and atfA (PubMed:26173180). Iron limitation acts as a third independent signal for terrein cluster induction via the iron response regulator hapX (PubMed:26173180). Finally, expression is under the control of the terrein cluster-specific transcription factor terR (PubMed:25852654). {ECO:0000269|PubMed:25852654, ECO:0000269|PubMed:26173180}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 1617; /note=O-(pantetheine 4'-phosphoryl)serine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00258; MOD_RES 1736; /note=O-(pantetheine 4'-phosphoryl)serine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00258 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 227,018 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:62972; RHEA:62973 |
| Cross Reference Brenda |