Detail Information for IndEnz0016000160
IED ID IndEnz0016000160
Enzyme Type ID tyrosinase000160
Protein Name FAD-dependent monooxygenase terC
EC 1.-.-.-
Terrein biosynthesis cluster protein terC
Gene Name terC ATEG_00143
Organism Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Enzyme Sequence MLRRAGVLEDVRRAGFVPKNWSFRKADGTPIVTIEDFAPSKSPDATTVLPIDTLGKILWCHALKNDHISVRWGCHVVDVGQDKDSAWVSVRENGGTEKIIKADFVCGCDGAASQVRKSLFGGEFRGKTWTDQMVATNVYYPFDEWGYDDLNTLIDPDDWHIVARLTKDGLWRVSYRQDGSKSLREVLDEHPARFEHLLPGHPKPDDYTILNISPYRLHQRSAEKYRVGRICLAADAAHLCNPWGGLGLTGGFADAIGLSECLIGIHMGRADHSILDKYDEVRRAIYWNFTDPISTSNFMRVSTSDPETILEKDPVMATFARMHEDSKIREDFDRVCSHPNIFVDLASLSQHIYGILHDFTQYYSTGKGQASL
Enzyme Length 372
Uniprot Accession Number Q0D1P1
Absorption
Active Site
Activity Regulation
Binding Site BINDING 76; /note=FAD; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; BINDING 215; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; BINDING 235; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6
Calcium Binding
catalytic Activity
DNA Binding
EC Number 1.-.-.-
Enzyme Function FUNCTION: FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of terrein, a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities (PubMed:24816227). The first step in the pathway is performed by the polyketide synthase terA that produces 4-hydroxy-6-methylpyranon (4-HMP), orsellinic acid (OA), and 2,3-dehydro-6-hydroxymellein (2,3-dehydro-6-HM) by condensing acetyl-CoA with two, three, or four malonyl-CoA units, respectively (PubMed:24816227). 4-HMP and OA are not pathway intermediates, but are rather shunt or side products (PubMed:24816227). 2,3-dehydro-6-HM is further converted to 6-hydroxymellein (6-HM) by the 6-hydroxymellein synthase terB (PubMed:24816227). The monooxygenases terC and terD, the multicopper oxidase terE and the Kelch-like protein terF are then involved in the transformation of 6-HM to terrein (PubMed:24816227). Even if they are co-regulated with the other terrein cluster genes, terH and terI seem to be dispensable for terrein production; whereas one or both of the 2 transporters terG or terJ are probably required for efficient secretion of metabolites (PubMed:24816227). {ECO:0000269|PubMed:24816227}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:24816227}.
nucleotide Binding NP_BIND 172..174; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6
Features Binding site (3); Chain (1); Nucleotide binding (1)
Keywords FAD;Flavoprotein;Monooxygenase;Oxidoreductase;Reference proteome
Interact With
Induction INDUCTION: Expression is induced by methionine (PubMed:26173180). Nitrogen starvation induces expression of terC and promotes terrein production during fruit infection, via regulation by areA and atfA (PubMed:26173180). Iron limitation acts as a third independent signal for terrein cluster induction via the iron response regulator hapX (PubMed:26173180). Finally, expression is under the control of the terrein cluster-specific transcription factor terR (PubMed:25852654). {ECO:0000269|PubMed:25852654, ECO:0000269|PubMed:26173180}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 41,918
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda