Detail Information for IndEnz0016000162
IED ID IndEnz0016000162
Enzyme Type ID tyrosinase000162
Protein Name FAD-dependent monooxygenase terD
EC 1.-.-.-
Terrein biosynthesis cluster protein terD
Gene Name terD ATEG_00142
Organism Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Enzyme Sequence MSSKFDVVICGSGTAGLAAATWLAQYGVDCKILESRSGPLDVGQADGIQVRSVEIFESFGMAEELLREAYHNIEVAFWGSNPTSSGMGIVRKRSAHATTPGLSHMPRVILNQARFNEMWLEAMRRRNGQEVDYGHKVTKVTVDEEKATDPDAYPVTIVAQKDGQEQVFEAKYCLASDGAHSAVRKSLGFNMVGETSDSVWGVMDVFPRTNFPDIRKQCIIQSDAGSIITIPREGGSMVRVYVELAAGTNAKEVTLEQIQNASRQVFHPYALDVADVWWWSAYPIGQRIADHFSKANRVFLTGDACHTHSPKAGQGMNVSLQDGYNIGWKLASVLKGHAGPELLETYVLERQKVADVLINWDKVWAKQMCSIAKEDGGVVDANGKIDFSEVFVKAEAFTAGLTVTYGDSIITQAGDSNQQAATNLKVGMRLPAAQVVRFCDAKVMKTVNALPSDGRWRIMIFPGDIRQPSASTRLAQLGTYLFSNHGPIRKYLPPGADIDSLIEVIVILSGERLEIQQDQIPDAFWPTTGKYRMRDLHKIYIDDETYHNGHGHAYDFYGIDPERGAVAIVRPDQYISKVLDMKNHEGISAFFEKFLQKKGQANGSLNSHDEWTLA
Enzyme Length 614
Uniprot Accession Number Q0D1P2
Absorption
Active Site
Activity Regulation
Binding Site BINDING 44; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; BINDING 137; /note=FAD; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; BINDING 282; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; BINDING 303; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; BINDING 319; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6
Calcium Binding
catalytic Activity
DNA Binding
EC Number 1.-.-.-
Enzyme Function FUNCTION: FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of terrein, a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities (PubMed:24816227). The first step in the pathway is performed by the polyketide synthase terA that produces 4-hydroxy-6-methylpyranon (4-HMP), orsellinic acid (OA), and 2,3-dehydro-6-hydroxymellein (2,3-dehydro-6-HM) by condensing acetyl-CoA with two, three, or four malonyl-CoA units, respectively (PubMed:24816227). 4-HMP and OA are not pathway intermediates, but are rather shunt or side products (PubMed:24816227). 2,3-dehydro-6-HM is further converted to 6-hydroxymellein (6-HM) by the 6-hydroxymellein synthase terB (PubMed:24816227). The monooxygenases terC and terD, the multicopper oxidase terE and the Kelch-like protein terF are then involved in the transformation of 6-HM to terrein (PubMed:24816227). Even if they are co-regulated with the other terrein cluster genes, terH and terI seem to be dispensable for terrein production; whereas one or both of the 2 transporters terG and terJ are probably required for efficient secretion of metabolites (PubMed:24816227). {ECO:0000269|PubMed:24816227}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:24816227}.
nucleotide Binding NP_BIND 6..35; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6; NP_BIND 239..241; /note=FAD; /evidence=ECO:0000250|UniProtKB:Q6SSJ6
Features Binding site (5); Chain (1); Glycosylation (3); Nucleotide binding (2); Signal peptide (1)
Keywords FAD;Flavoprotein;Glycoprotein;Monooxygenase;Oxidoreductase;Reference proteome;Signal
Interact With
Induction INDUCTION: Expression is under the control of the terrein cluster-specific transcription factor terR (PubMed:25852654). {ECO:0000269|PubMed:25852654}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 67,859
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda