| IED ID | IndEnz0016000166 |
| Enzyme Type ID | tyrosinase000166 |
| Protein Name |
5,6-dihydroxyindole-2-carboxylic acid oxidase DHICA oxidase EC 1.14.18.- Tyrosinase-related protein 1 TRP-1 TRP1 |
| Gene Name | TYRP1 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MQLPMLLLVSLPLLLNMFKPAEAQFPRQCATIESLRSGMCCPDYFPVFGPGSDQCGVSTGRGRCVQVTVDSRPHGPQYIHDGRDDREQWPIRFFNQTCRCNGNFSGYNCGSCRPGWTGPTCSQQINIVRRNLLDLSTEERRRFVNALHQAKVTIHPDIVIATRRREEIFGPDGNTPQFENISIYNYFVWSHYYSVRKTFLGAGQQSFERVDFSHEGPAFVTWHRYHLLQLERDMQNMLQDSTFGLPYWNFATGQNTCDICSDDLMGARSNFDVSLISQNSIFSTWRVLCESIEDYDSLGTICNSTEGGPIRRNPAGNVARPMVQRLPEPEDVPQCLEVGIFDTPPFYSNSTDSFRNTVEGYSDPSGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYSADISTYPLENAPIGHNREYNMVPFWPPVTNNEMFVTAPENLGYSYDIEWPGPLRVTEMITIAIVTALVLVAIIFAAAACIVRAKKNRDELHQPLLTDQYQHYSDDYDGIATPSQSVV |
| Enzyme Length | 535 |
| Uniprot Accession Number | O57405 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.14.18.- |
| Enzyme Function | FUNCTION: Plays a role in melanin biosynthesis. Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. Also to a lower extent, capable of hydroxylating tyrosine and producing melanin. {ECO:0000250|UniProtKB:P07147}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis; melanin biosynthesis. {ECO:0000250|UniProtKB:P17643}. |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (7); Glycosylation (6); Metal binding (6); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Copper;Disulfide bond;Glycoprotein;Melanin biosynthesis;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Melanosome membrane {ECO:0000250|UniProtKB:P07147}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P07147}. Melanosome {ECO:0000250|UniProtKB:P07147}. Note=Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. {ECO:0000250|UniProtKB:P07147}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 30457703; |
| Motif | |
| Gene Encoded By | |
| Mass | 60,595 |
| Kinetics | |
| Metal Binding | METAL 191; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 214; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 223; /note=Zinc 1; binds zinc A in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 376; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 380; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643; METAL 403; /note=Zinc 2; binds zinc B in the binuclear zinc-binding site; /evidence=ECO:0000250|UniProtKB:P17643 |
| Rhea ID | |
| Cross Reference Brenda |