Detail Information for IndEnz0016000184
IED ID IndEnz0016000184
Enzyme Type ID tyrosinase000184
Protein Name Phenoloxidase subunit 2
EC 1.14.18.1
proPO-p2
Gene Name ppo
Organism Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Amphiesmenoptera Lepidoptera (butterflies and moths) Glossata Neolepidoptera Heteroneura Ditrysia Obtectomera Bombycoidea (hawk-moths) Sphingidae (hawkmoths) Sphinginae (small-eyed sphinx moth) Sphingini Manduca Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Enzyme Sequence MADIFDSFELLYDRPGEPMINTKGEDKVLFELTEQFLTPEYANNGLELNNRFGDEEEVSRKIILKNLDKIPEFPKAKQLPNDADFSLFLPSHQEMANEVIDVLMSVTENQLQELLSTCVYARINLNPQLFNYCYTVAIMHRRDTGKVRVQNYAEIFPAKFLDSQVFTQAREAAAVIPKTIPRTPIIIPRDYTATDLEEEHRLAYWREDLGINLHHWHWHLVYPFSASDEKIVAKDRRGELFFYMHQQIIARYNCERLCNSLKRVKKFSDWREPIPEAYYPKLDSLTSARGWPPRQAGMRWQDLKRPVDGLNVTIDDMERYRRNIEEAIATGNVILPDKSTKKLDIDMLGNMMEASVLSPNRDLYGSIHNNMHSFSAYMHDPEHRYLESFGVIADEATTMRDPFFYRVHAWVDDIFQSFKEAPHNVRPYSRSQLENPGVQVTSVAVESAGGQQNVLNTFWMQSDVNLSKGLDFSDRGPVYARFTHLNHRPFRYVIKANNTASARRTTVRIFIAPKTDERNLPWALSDQRKMFIEMDRFVVPLSAGENTITRQSTESSLTIPFEQTFRDLSIQGSDPRRSELAAFNYCGCGWPQHMLVPKGTVGGVAYQLFVMLSNYELDKIEQPDGRELSCVEASMFCGLKDKKYPDARPMGYPFDRPSNSATNIEDFSAMSNMGLQDIVIKLSDVTEPNPRNPPA
Enzyme Length 695
Uniprot Accession Number Q25519
Absorption
Active Site ACT_SITE 353; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q8MZM3
Activity Regulation ACTIVITY REGULATION: Activated by immulectin and lipopolysaccharide. {ECO:0000269|PubMed:10436935}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1;
DNA Binding
EC Number 1.14.18.1
Enzyme Function FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone. Binds to the surface of hemocytes and is involved in hemocyte melanization. {ECO:0000269|PubMed:16291091, ECO:0000305}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Beta strand (20); Chain (1); Disulfide bond (2); Helix (33); Initiator methionine (1); Metal binding (6); Turn (8)
Keywords 3D-structure;Copper;Direct protein sequencing;Disulfide bond;Melanin biosynthesis;Metal-binding;Monooxygenase;Oxidoreductase;Secreted
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9474780}.
Modified Residue
Post Translational Modification PTM: The N-terminus is blocked. {ECO:0000269|PubMed:9474780}.
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 3HHS;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 80,061
Kinetics
Metal Binding METAL 215; /note=Copper A; /evidence=ECO:0000269|PubMed:19805072; METAL 219; /note=Copper A; /evidence=ECO:0000269|PubMed:19805072; METAL 245; /note=Copper A; /evidence=ECO:0000269|PubMed:19805072; METAL 368; /note=Copper B; /evidence=ECO:0000269|PubMed:19805072; METAL 372; /note=Copper B; /evidence=ECO:0000269|PubMed:19805072; METAL 408; /note=Copper B; /evidence=ECO:0000269|PubMed:19805072
Rhea ID RHEA:34287; RHEA:18117
Cross Reference Brenda