Detail Information for IndEnz0016000186
IED ID IndEnz0016000186
Enzyme Type ID tyrosinase000186
Protein Name Kelch-like protein terF
Terrein biosynthesis cluster protein terF
Gene Name terF ATEG_00140
Organism Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Enzyme Sequence MPPTISDDTTVRSFTCAQPLGRAGYELIIRQMLHRFLSDLHTTKALLDRSLQATVNHAAGPVRSLLTIITVGFYTLLNWCSLGSYHTTKMISTVVTGLLLLASGCEALPTNQTPLPGKWSTLPNITLNGVEYPRQEHAAALVGDEIFVLGGILPWDGKEYATTNIVQKYNMITGTWTETAPMPAALNHANVAVVDGKIYYLGGLEAVDETYWNATGKSAVYDPATDEWTVLPSMPEGREIGSAATVVVDDTIYLPGGLAYTNITYDQEGTVSRFSSYNVRTQEWTTLPDLPAPRDHAGKGIYRDMLYILGGREFGNKNVVSTVFGFNLTSQQWATAFEPMPIARGGVASATIGSLIFTAGGEGDRRTPTAVFPEMQAYDAATNTWVDYADMPLPVHGSDAVVYKGEIVIPGGGIVTGATLTPVVQTFQPPLPDSGEKSMPLMVMVYRVVFDLPWMLFKR
Enzyme Length 459
Uniprot Accession Number Q0D1P4
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Kelch-like protein; part of the gene cluster that mediates the biosynthesis of terrein, a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities (PubMed:24816227). The first step in the pathway is performed by the polyketide synthase terA that produces 4-hydroxy-6-methylpyranon (4-HMP), orsellinic acid (OA), and 2,3-dehydro-6-hydroxymellein (2,3-dehydro-6-HM) by condensing acetyl-CoA with two, three, or four malonyl-CoA units, respectively (PubMed:24816227). 4-HMP and OA are not pathway intermediates, but are rather shunt or side products (PubMed:24816227). 2,3-dehydro-6-HM is further converted to 6-hydroxymellein (6-HM) by the 6-hydroxymellein synthase terB (PubMed:24816227). The monooxygenases terC and terD, the multicopper oxidase terE and the Kelch-like protein terF are then involved in the transformation of 6-HM to terrein (PubMed:24816227). Even if they are co-regulated with the other terrein cluster genes, terH and terI seem to be dispensable for terrein production; whereas one or both of the 2 transporters terG and terJ are probably required for efficient secretion of metabolites (PubMed:24816227). {ECO:0000269|PubMed:24816227}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:24816227}.
nucleotide Binding
Features Chain (1); Repeat (6)
Keywords Kelch repeat;Reference proteome;Repeat
Interact With
Induction INDUCTION: Expression is under the control of the terrein cluster-specific transcription factor terR (PubMed:25852654). {ECO:0000269|PubMed:25852654}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 49,873
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda