IED ID | IndEnz0016000186 |
Enzyme Type ID | tyrosinase000186 |
Protein Name |
Kelch-like protein terF Terrein biosynthesis cluster protein terF |
Gene Name | terF ATEG_00140 |
Organism | Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
Enzyme Sequence | MPPTISDDTTVRSFTCAQPLGRAGYELIIRQMLHRFLSDLHTTKALLDRSLQATVNHAAGPVRSLLTIITVGFYTLLNWCSLGSYHTTKMISTVVTGLLLLASGCEALPTNQTPLPGKWSTLPNITLNGVEYPRQEHAAALVGDEIFVLGGILPWDGKEYATTNIVQKYNMITGTWTETAPMPAALNHANVAVVDGKIYYLGGLEAVDETYWNATGKSAVYDPATDEWTVLPSMPEGREIGSAATVVVDDTIYLPGGLAYTNITYDQEGTVSRFSSYNVRTQEWTTLPDLPAPRDHAGKGIYRDMLYILGGREFGNKNVVSTVFGFNLTSQQWATAFEPMPIARGGVASATIGSLIFTAGGEGDRRTPTAVFPEMQAYDAATNTWVDYADMPLPVHGSDAVVYKGEIVIPGGGIVTGATLTPVVQTFQPPLPDSGEKSMPLMVMVYRVVFDLPWMLFKR |
Enzyme Length | 459 |
Uniprot Accession Number | Q0D1P4 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | |
Enzyme Function | FUNCTION: Kelch-like protein; part of the gene cluster that mediates the biosynthesis of terrein, a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities (PubMed:24816227). The first step in the pathway is performed by the polyketide synthase terA that produces 4-hydroxy-6-methylpyranon (4-HMP), orsellinic acid (OA), and 2,3-dehydro-6-hydroxymellein (2,3-dehydro-6-HM) by condensing acetyl-CoA with two, three, or four malonyl-CoA units, respectively (PubMed:24816227). 4-HMP and OA are not pathway intermediates, but are rather shunt or side products (PubMed:24816227). 2,3-dehydro-6-HM is further converted to 6-hydroxymellein (6-HM) by the 6-hydroxymellein synthase terB (PubMed:24816227). The monooxygenases terC and terD, the multicopper oxidase terE and the Kelch-like protein terF are then involved in the transformation of 6-HM to terrein (PubMed:24816227). Even if they are co-regulated with the other terrein cluster genes, terH and terI seem to be dispensable for terrein production; whereas one or both of the 2 transporters terG and terJ are probably required for efficient secretion of metabolites (PubMed:24816227). {ECO:0000269|PubMed:24816227}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:24816227}. |
nucleotide Binding | |
Features | Chain (1); Repeat (6) |
Keywords | Kelch repeat;Reference proteome;Repeat |
Interact With | |
Induction | INDUCTION: Expression is under the control of the terrein cluster-specific transcription factor terR (PubMed:25852654). {ECO:0000269|PubMed:25852654}. |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 49,873 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |