| IED ID | IndEnz0016000192 |
| Enzyme Type ID | tyrosinase000192 |
| Protein Name |
Phenoloxidase subunit 1 EC 1.14.18.1 PO-P1 Fragment |
| Gene Name | |
| Organism | Simulium damnosum (Black fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Nematocera Culicomorpha Chironomoidea Simuliidae (black flies) Simuliinae Simuliini Simulium Edwardsellum damnosum complex Simulium damnosum (Black fly) |
| Enzyme Sequence | MRDPFFYRWHSYIDDIFQEHKERLRPYTEAQLNFNGITVTGVQVAPERGPTNTFQTSWQQSDVDLSRGMDFVAPRGNVTARFTHLNHTPFTYSIQVNNSSGAQRMGMVRIFLAPKTDERGNEMLFRDQRLMMIEMDKFVVSMRPGQNTIRRRSTESTVTIPFERTFRSLEESRPDQTTDAQQQFNFCGCGWPHHM |
| Enzyme Length | 195 |
| Uniprot Accession Number | P85046 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; CATALYTIC ACTIVITY: Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; |
| DNA Binding | |
| EC Number | 1.14.18.1 |
| Enzyme Function | FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone. {ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (3); Metal binding (1); Non-terminal residue (2) |
| Keywords | Copper;Glycoprotein;Melanin biosynthesis;Metal-binding;Monooxygenase;Oxidoreductase;Secreted |
| Interact With | |
| Induction | INDUCTION: By infection with O.dukei and O.ochengi. {ECO:0000269|PubMed:9225772}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 22,867 |
| Kinetics | |
| Metal Binding | METAL 10; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q27451 |
| Rhea ID | RHEA:34287; RHEA:18117 |
| Cross Reference Brenda |