Detail Information for IndEnz0016000193
IED ID IndEnz0016000193
Enzyme Type ID tyrosinase000193
Protein Name Polyphenol oxidase D, chloroplastic
PPO
EC 1.10.3.1
Catechol oxidase
Gene Name
Organism Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Enzyme Sequence MASLCSNSSTTSLKTPFTSLGSTPKPCQLFLHGKRNKAFKVSCKVTNTNGNQDETNSVDRRNVLLGLGGLYGVANAIPLAASAAPTPPPDLSSCSIARIDENQVVSYSCCAPKPDDMEKVPYYKFPSMTKLRVRQPAHEADEEYIAKYNVSVTKMRDLDKTQPLNPIGFKQQANIHCAYCNGAYRIGGKELQVHNSWLFFPFHRWYLYFYESNAGKLIDDPTFALPYWNWDHPKGMRFPAMYDREGTFLFDETRDQSHRNGTVIDLGFFGNEVETTQLQMMSNNLTLMYRQMVTNAPCPRMFFGDLMISGITLNSPGTIENIPHGPVHIWSGTVRGSTLPNGAISKRGEYGHFYSAGLDPVFFCHHSNVDRMWSEWKATGGKRTDITHKDWLNSEFFFYDENENPYRVKVRDCLDTKKMGYDYKPMRTPWRNFKPLTKASAGKVNTSSIPPVSQAFPLAKLDKAVSFSINRPTSSRTPQEKNAQEEMLTFNSIRYDNRGYIRFDVFLNVDNNVNANELDKAEFAGSYTSLPHVHRAGETNHIATVDFQLAITELLEDIGLEDEDTIAVTLVPKRGGEGISIENATISLADC
Enzyme Length 591
Uniprot Accession Number Q08306
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O; Xref=Rhea:RHEA:21632, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17253, ChEBI:CHEBI:18135; EC=1.10.3.1;
DNA Binding
EC Number 1.10.3.1
Enzyme Function FUNCTION: Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Cross-link (1); Disulfide bond (2); Metal binding (6); Transit peptide (1)
Keywords Chloroplast;Copper;Disulfide bond;Metal-binding;Oxidoreductase;Plastid;Reference proteome;Thioether bond;Thylakoid;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 66,529
Kinetics
Metal Binding METAL 176; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 194; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 203; /note=Copper A; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 324; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 328; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19; METAL 366; /note=Copper B; /evidence=ECO:0000250|UniProtKB:Q9ZP19
Rhea ID RHEA:21632
Cross Reference Brenda