| IED ID | IndEnz0016000230 |
| Enzyme Type ID | tyrosinase000230 |
| Protein Name |
Tyrosine phenol-lyase EC 4.1.99.2 Beta-tyrosinase |
| Gene Name | tpl |
| Organism | Citrobacter freundii |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Citrobacter Citrobacter freundii complex Citrobacter freundii |
| Enzyme Sequence | MNYPAEPFRIKSVETVSMIPRDERLKKMQEAGYNTFLLNSKDIYIDLLTDSGTNAMSDKQWAGMMMGDEAYAGSENFYHLERTVQELFGFKHIVPTHQGRGAENLLSQLAIKPGQYVAGNMYFTTTRYHQEKNGAVFVDIVRDEAHDAGLNIAFKGDIDLKKLQKLIDEKGAENIAYICLAVTVNLAGGQPVSMANMRAVRELTEAHGIKVFYDATRCVENAYFIKEQEQGFENKSIAEIVHEMFSYADGCTMSGKKDCLVNIGGFLCMNDDEMFSSAKELVVVYEGMPSYGGLAGRDMEAMAIGLREAMQYEYIEHRVKQVRYLGDKLKAAGVPIVEPVGGHAVFLDARRFCEHLTQDEFPAQSLAASIYVETGVRSMERGIISAGRNNVTGEHHRPKLETVRLTIPRRVYTYAHMDVVADGIIKLYQHKEDIRGLKFIYEPKQLRFFTARFDYI |
| Enzyme Length | 456 |
| Uniprot Accession Number | P31013 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate; Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377, ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2; |
| DNA Binding | |
| EC Number | 4.1.99.2 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (19); Chain (1); Helix (23); Modified residue (1); Sequence conflict (1); Turn (8) |
| Keywords | 3D-structure;Direct protein sequencing;Lyase;Pyridoxal phosphate |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 257; /note=N6-(pyridoxal phosphate)lysine |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (22) |
| Cross Reference PDB | 1TPL; 2EZ1; 2EZ2; 2TPL; 2VLF; 2VLH; 2YCN; 2YCP; 2YCT; 2YHK; 6DUR; 6DVX; 6DXV; 6DYT; 6DZ5; 6ECG; 6MLS; 6MME; 6MO3; 6MPD; 6MQQ; 6NV8; |
| Mapped Pubmed ID | 16768450; 18715865; 21899319; 30260636; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,500 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21704 |
| Cross Reference Brenda | 4.1.99.2; |