| IED ID | IndEnz0017000010 |
| Enzyme Type ID | manganese peroxidase000010 |
| Protein Name |
Dye-decolorizing peroxidase msp1 EC 1.11.1.19 EC 1.11.1.7 Peroxidase 1 MsP1 |
| Gene Name | msp1 |
| Organism | Mycetinis scorodonius (Garlic mushroom) (Marasmius scorodonius) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Omphalotaceae Mycetinis Mycetinis scorodonius (Garlic mushroom) (Marasmius scorodonius) |
| Enzyme Sequence | MKLFSASVFAAIIASHYASATAHIRAPNVKPRRTNSLLTAPPQQPPLPSAQQAASASSSAGLNLTDIQGDILIGMKKNKELFFFFSITDAATFKAKLGSDILELITSTNQLLAVATQPITAVNVAFSSTGLKALGITDDLKDPVFEAGMLSNAVSDLSDPGTGNWVPGFVGTSVHGVFLLASDTIDNVNTELANIQTILNGSITEIHRLQGEARPGDQQGHEHFGFMDGISNPAVDGFTPPAEIRPGQALIPPGIMLLGEANDTFQNDRPPWAKDGSFLVFRQMQQRAPEFNKFLQDHALNMPNMTSEQGADLLGARIVGRWKSDAPIDLTPLVDDPVLAADNQRNNNFDFSDATNQTRCPFSAHIRKANPRGDLGGINKFPNQHIIRAGIPYGPEVTDAEKASNSSSTDPSLERGLAFVAYQSNIQNGFVFLQKNWVDNTNFFRPGTGVDPLIGTNSRNSGTDAPNTPRVVSGLDPNNATSTIEIGIDFVVSRGGEYFFSPSLSAIRTVLSV |
| Enzyme Length | 513 |
| Uniprot Accession Number | B0BK71 |
| Absorption | BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Abs(max)=406 nm {ECO:0000269|PubMed:23111597}; |
| Active Site | ACT_SITE 228; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:I2DBY1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 H2O2 + Reactive Blue 5 = 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate + 2 H(+) + 2 H2O + phthalate; Xref=Rhea:RHEA:28086, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:17563, ChEBI:CHEBI:63950, ChEBI:CHEBI:63955, ChEBI:CHEBI:64278; EC=1.11.1.19; Evidence={ECO:0000269|PubMed:23111597}; CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; Evidence={ECO:0000269|PubMed:23111597}; |
| DNA Binding | |
| EC Number | 1.11.1.19; 1.11.1.7 |
| Enzyme Function | FUNCTION: Manganese-independent peroxidase that is able to convert a large number of compounds, but its physiological substrate is not known (PubMed:23111597). In addition to classic peroxidase substrates (e.g. 2,6-dimethoxyphenol), oxidizes dyes such as Reactive Blue 5 (PubMed:23111597). Also degrades beta-carotene (PubMed:18038130). {ECO:0000269|PubMed:18038130, ECO:0000269|PubMed:23111597}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (1); Propeptide (1); Region (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Heme;Iron;Metal-binding;Oxidoreductase;Peroxidase;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18038130}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,972 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5 uM for H(2)O(2) {ECO:0000269|PubMed:23111597}; |
| Metal Binding | METAL 365; /note=Iron (heme axial ligand); via tele nitrogen; /evidence=ECO:0000250|UniProtKB:I2DBY1 |
| Rhea ID | RHEA:28086; RHEA:56136 |
| Cross Reference Brenda |