| IED ID | IndEnz0017000027 |
| Enzyme Type ID | manganese peroxidase000027 |
| Protein Name |
Coproheme decarboxylase EC 1.3.98.5 Coproheme III oxidative decarboxylase Hydrogen peroxide-dependent heme synthase |
| Gene Name | chdC GT50_08830 |
| Organism | Geobacillus stearothermophilus (strain DSM 13240 / CIP 106956 / 10) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Geobacillus Geobacillus stearothermophilus (Bacillus stearothermophilus) Geobacillus stearothermophilus (strain DSM 13240 / CIP 106956 / 10) |
| Enzyme Sequence | MSEAAQTLDGWYCLHDFRTIDWSAWKTLPNEEREAAISEFLALVDQWETTESEKQGSHAVYTIVGQKADILFMILRPTLDELHEIETALNKTKLADYLLPAYSYVSVVELSNYLASGSEDPYQIPEVRRRLYPILPKTNYICFYPMDKRRQGNDNWYMLSMEQRRELMRAHGMTGRKYAGKVTQIITGSVGLDDFEWGVTLFSDDALQFKKLVYEMRFDEVSARFGEFGSFFVGTRLPMENVSSFFHV |
| Enzyme Length | 248 |
| Uniprot Accession Number | A0A0K2H9D8 |
| Absorption | |
| Active Site | ACT_SITE 144; /evidence=ECO:0000255|HAMAP-Rule:MF_01442 |
| Activity Regulation | |
| Binding Site | BINDING 130; /note="Fe-coproporphyrin III"; /evidence="ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000305|PubMed:27936663"; BINDING 144; /note="Fe-coproporphyrin III"; /evidence="ECO:0000305|PubMed:27936663"; BINDING 148; /note="Fe-coproporphyrin III"; /evidence="ECO:0000305|PubMed:27936663"; BINDING 184; /note="Fe-coproporphyrin III"; /evidence="ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000305|PubMed:27936663"; BINDING 222; /note="Fe-coproporphyrin III"; /evidence="ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000305|PubMed:27936663" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Fe-coproporphyrin III + 2 H(+) + 2 H2O2 = 2 CO2 + 4 H2O + heme b; Xref=Rhea:RHEA:56516, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:16526, ChEBI:CHEBI:60344, ChEBI:CHEBI:68438; EC=1.3.98.5; Evidence={ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:56517; Evidence={ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663}; CATALYTIC ACTIVITY: Reaction=Fe-coproporphyrin III + H(+) + H2O2 = CO2 + 2 H2O + harderoheme III; Xref=Rhea:RHEA:57940, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:16526, ChEBI:CHEBI:68438, ChEBI:CHEBI:142463; Evidence={ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57941; Evidence={ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663}; CATALYTIC ACTIVITY: Reaction=H(+) + H2O2 + harderoheme III = CO2 + 2 H2O + heme b; Xref=Rhea:RHEA:57944, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:16526, ChEBI:CHEBI:60344, ChEBI:CHEBI:142463; Evidence={ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57945; Evidence={ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663}; |
| DNA Binding | |
| EC Number | 1.3.98.5 |
| Enzyme Function | FUNCTION: Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:27936663). Catalyzes the decarboxylation of Fe-coproporphyrin III (coproheme) to heme b (protoheme IX), the last step of the pathway (PubMed:27936663). The reaction occurs in a stepwise manner with a three-propionate harderoheme intermediate (PubMed:27936663). {ECO:0000269|PubMed:27936663}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Porphyrin-containing compound metabolism; protoheme biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01442}. |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (9); Binding site (5); Chain (1); Helix (12); Metal binding (1); Region (1); Turn (2) |
| Keywords | 3D-structure;Heme;Heme biosynthesis;Iron;Metal-binding;Oxidoreductase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5T2K; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,787 |
| Kinetics | |
| Metal Binding | METAL 171; /note="Iron (Fe-coproporphyrin III axial ligand)"; /evidence="ECO:0000255|HAMAP-Rule:MF_01442, ECO:0000269|PubMed:27936663" |
| Rhea ID | RHEA:56516; RHEA:56517; RHEA:57940; RHEA:57941; RHEA:57944; RHEA:57945 |
| Cross Reference Brenda | 1.3.98.5; |