| IED ID | IndEnz0018000004 |
| Enzyme Type ID | peroxidase000004 |
| Protein Name |
Glutathione peroxidase 2 GPx-2 GSHPx-2 EC 1.11.1.9 Gastrointestinal glutathione peroxidase Glutathione peroxidase-gastrointestinal GPx-GI GSHPx-GI Glutathione peroxidase-related protein 2 GPRP-2 |
| Gene Name | GPX2 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLUGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI |
| Enzyme Length | 190 |
| Uniprot Accession Number | P18283 |
| Absorption | |
| Active Site | ACT_SITE 40 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O; Xref=Rhea:RHEA:62632, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:57925, ChEBI:CHEBI:58297; Evidence={ECO:0000269|PubMed:8428933};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62633; Evidence={ECO:0000269|PubMed:8428933}; CATALYTIC ACTIVITY: Reaction=2 glutathione + H2O2 = glutathione disulfide + 2 H2O; Xref=Rhea:RHEA:16833, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:57925, ChEBI:CHEBI:58297; EC=1.11.1.9; Evidence={ECO:0000269|PubMed:8428933};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16834; Evidence={ECO:0000269|PubMed:8428933}; CATALYTIC ACTIVITY: Reaction=2 glutathione + tert-butyl hydroperoxide = glutathione disulfide + H2O + tert-butanol; Xref=Rhea:RHEA:69412, ChEBI:CHEBI:15377, ChEBI:CHEBI:45895, ChEBI:CHEBI:57925, ChEBI:CHEBI:58297, ChEBI:CHEBI:64090; Evidence={ECO:0000269|PubMed:8428933};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69413; Evidence={ECO:0000269|PubMed:8428933}; CATALYTIC ACTIVITY: Reaction=cumene hydroperoxide + 2 glutathione = 2-phenylpropan-2-ol + glutathione disulfide + H2O; Xref=Rhea:RHEA:69651, ChEBI:CHEBI:15377, ChEBI:CHEBI:57925, ChEBI:CHEBI:58297, ChEBI:CHEBI:78673, ChEBI:CHEBI:131607; Evidence={ECO:0000269|PubMed:8428933};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69652; Evidence={ECO:0000269|PubMed:8428933}; |
| DNA Binding | |
| EC Number | 1.11.1.9 |
| Enzyme Function | FUNCTION: Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides (PubMed:8428933). Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors (PubMed:8428933). {ECO:0000269|PubMed:8428933}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (8); Chain (1); Helix (9); Natural variant (4); Non-standard residue (1); Sequence conflict (2); Turn (1) |
| Keywords | 3D-structure;Cytoplasm;Oxidoreductase;Peroxidase;Reference proteome;Selenocysteine |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:8428933}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2HE3; |
| Mapped Pubmed ID | 10549853; 11115402; 11811519; 12751789; 15923610; 16446369; 16794261; 17277236; 17937616; 18056462; 18479189; 18483336; 18676680; 19047153; 19161995; 19170196; 19573080; 19625176; 19692168; 19773279; 19913121; 20200426; 20628086; 20813000; 21988832; 22683372; 22758632; 23934683; 24562575; 2501828; 25261240; 25813210; 27388201; 28453466; 28631563; 28635398; 28916653; 30652380; 31765890; 31774184; 32215178; 33622868; 34954079; 35193955; 6816802; |
| Motif | |
| Gene Encoded By | |
| Mass | 21,954 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:62632; RHEA:62633; RHEA:16833; RHEA:16834; RHEA:69412; RHEA:69413; RHEA:69651; RHEA:69652 |
| Cross Reference Brenda | 1.11.1.9; |