| IED ID | IndEnz0018000007 |
| Enzyme Type ID | peroxidase000007 |
| Protein Name |
Eosinophil peroxidase EPO EC 1.11.1.7 Cleaved into: Eosinophil peroxidase light chain; Eosinophil peroxidase heavy chain |
| Gene Name | Epx Eper |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MMQQLLALVGALATLILTQHAEGTAPASPSPVEISVLRDCIAEAKLLVDTAYNHTQKSIMQRLRSGSASPMDLLAYFKQPVAATRRVVQAADYMHVALGLLEERLQPRGSRPFNATDVLTEPQLRLLSQASGCALQDQAERCSNKYRTITGRCNNKKHPWLGASNQALARWLPAEYEDHRSLPFGWTPGKRRNGFLLPLVRDVSNQIVRFPSKKLTSDRGRALMFMQWGQFIDHDLDFSPESPARVAFSMGVDCEKTCAQLPPCFPIKIPRNDPRIKNQRDCIPFFRSAPACPQNRNKVRNQINALTSFVDASMVYGSEVTLALRLRNRTNFLGLLATNQRFQDNGRALLPFDNLHEDPCLLTNRSARIPCFLAGDTRSSETPKLTALHTLFVREHNRLAAELRRLNPHWSGDKLYNEARKIVGAMVQIITYRDFLPLVLGRARIRRTLGPYRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYRASAPNSHVPLSSVFFASWRIIHEGGIDPILRGLMATPAKLNRQDSMLVDELRDKLFQQVRRIGLDLAALNMQRSRDHGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLRLYKTPDNIDIWVGAIAEPLLPGARVGPLLACLFENQFRRARDGDRFWWQKWGVFTKRQRKALRRISLSRIVCDNTGITTVSRDIFRANIYPQGFVSCSRIPKLNLSAWRGK |
| Enzyme Length | 716 |
| Uniprot Accession Number | P49290 |
| Absorption | |
| Active Site | ACT_SITE 234; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298 |
| Activity Regulation | |
| Binding Site | BINDING 233; /note="Heme b; covalent, via 2 links"; /evidence="ECO:0000250"; BINDING 381; /note="Heme b; covalent, via 2 links"; /evidence="ECO:0000250" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; |
| DNA Binding | |
| EC Number | 1.11.1.7 |
| Enzyme Function | FUNCTION: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. {ECO:0000250, ECO:0000269|PubMed:18694936}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (2); Disulfide bond (6); Erroneous initiation (1); Glycosylation (5); Metal binding (6); Modified residue (1); Propeptide (1); Sequence conflict (4); Signal peptide (1); Site (1) |
| Keywords | Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Nitration;Oxidoreductase;Peroxidase;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils. |
| Modified Residue | MOD_RES 489; /note=3'-nitrotyrosine; /evidence=ECO:0000250|UniProtKB:P11678 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10770291; 11466391; 11711534; 14681479; 14742524; 15100311; 15520881; 16141072; 16297853; 16299347; 16926417; 21267068; 21454806; 21482685; 21677750; 22131328; 23630390; 23736699; 24266710; 24626328; 25429065; 26147683; 27049514; 27587397; 28302646; 28515227; 28566277; 28703297; 29281125; 29601103; 29633338; 29970473; 30271964; 30285291; 30397031; 30565999; 30917956; 31098414; 31481482; 33020661; 33598715; 33662369; 33792561; 34616019; 34717799; 9107693; |
| Motif | |
| Gene Encoded By | |
| Mass | 81,380 |
| Kinetics | |
| Metal Binding | METAL 235; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; METAL 307; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; METAL 309; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; METAL 311; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; METAL 313; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; METAL 475; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00298 |
| Rhea ID | RHEA:56136 |
| Cross Reference Brenda |