| IED ID | IndEnz0018000019 |
| Enzyme Type ID | peroxidase000019 |
| Protein Name |
Catalase-peroxidase CP EC 1.11.1.21 Hydroperoxidase I HPI Peroxidase/catalase |
| Gene Name | katG b3942 JW3914 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVDLLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIRMAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWADLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLGATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAGPTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLFKYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRRFLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDIIDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVRALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDIEMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFDGSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADLVFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL |
| Enzyme Length | 726 |
| Uniprot Accession Number | P13029 |
| Absorption | |
| Active Site | ACT_SITE 106; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_01961 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Displays also NADH oxidase, INH lyase and isonicotinoyl-NAD synthase activity. {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:23416055, ECO:0000269|PubMed:374409}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.25 for the peroxidase reaction and 7.5 for the catalase reaction. {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Compositional bias (1); Cross-link (2); Helix (16); Metal binding (1); Region (1); Sequence conflict (1); Site (1); Turn (5) |
| Keywords | 3D-structure;Direct protein sequencing;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | INDUCTION: By hydrogen peroxide. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked.; PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_01961}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1U2J; 1U2K; 1U2L; |
| Mapped Pubmed ID | 11257507; 11440837; 11479716; 12589799; 14659082; 15004283; 15690043; 15878120; 16183297; 16606699; 16858726; 19440203; 2192882; 3038676; 3049591; 3298208; 3543208; 7023475; 7775420; 8779563; 8990289; |
| Motif | |
| Gene Encoded By | |
| Mass | 80,024 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=35 mM for H(2)O(2) for the catalase reaction (at pH 5.5-6.0) {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; KM=4.2 mM for H(2)O(2) for the catalase reaction (at pH 7.0) {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; KM=3.9 mM for H(2)O(2) for the catalase reaction (at pH 7.5) {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; KM=60 uM for H(2)O(2) for the peroxidase reaction {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; KM=24 uM for ABTS for the peroxidase reaction {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; Vmax=3730 umol/min/mg enzyme for H(2)O(2) for the catalase reaction (at pH 5.5-6.0) {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; Vmax=2220 umol/min/mg enzyme for H(2)O(2) for the catalase reaction (at pH 7.0) {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; Vmax=18 umol/min/mg enzyme for ABTS for the peroxidase reaction {ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:374409}; |
| Metal Binding | METAL 267; /note=Iron (heme b axial ligand) |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda |