| IED ID | IndEnz0018000022 |
| Enzyme Type ID | peroxidase000022 |
| Protein Name |
Versatile peroxidase VPL2 EC 1.11.1.16 Versatile liquid phase peroxidase 2 |
| Gene Name | vpl2 |
| Organism | Pleurotus eryngii (Boletus of the steppes) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Pleurotaceae Pleurotus Pleurotus eryngii (Boletus of the steppes) |
| Enzyme Sequence | MSFKTLSALALALGAAVQFASAAVPLVQKRATCDDGRTTANAACCILFPILDDIQENLFDGAQCGEEVHESLRLTFHDAIGFSPTLGGGGADGSIIAFDTIETNFPANAGIDEIVSAQKPFVAKHNISAGDFIQFAGAVGVSNCPGGVRIPFFLGRPDAVAASPDHLVPEPFDSVDSILARMGDAGFSPVEVVWLLASHSIAAADKVDPSIPGTPFDSTPGVFDSQFFIETQLKGRLFPGTADNKGEAQSPLQGEIRLQSDHLLARDPQTACEWQSMVNNQPKIQNRFAATMSKMALLGQDKTKLIDCSDVIPTPPALVGAAHLPAGFSLSDVEQACAATPFPALTADPGPVTSVPPVPGS |
| Enzyme Length | 361 |
| Uniprot Accession Number | O94753 |
| Absorption | |
| Active Site | ACT_SITE 77; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000255|PROSITE-ProRule:PRU10012"; ACT_SITE 194; /note="Tryptophan radical intermediate"; /evidence="ECO:0000269|PubMed:16246366" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = glycolaldehyde + guaiacol + H2O + vanillin; Xref=Rhea:RHEA:22396, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17071, ChEBI:CHEBI:18346, ChEBI:CHEBI:28591, ChEBI:CHEBI:53650; EC=1.11.1.16; Evidence={ECO:0000269|PubMed:12884090, ECO:0000269|PubMed:16246366, ECO:0000269|PubMed:9987124}; CATALYTIC ACTIVITY: Reaction=2 H(+) + H2O2 + 2 Mn(2+) = 2 H2O + 2 Mn(3+); Xref=Rhea:RHEA:22776, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:29035, ChEBI:CHEBI:29041; EC=1.11.1.16; Evidence={ECO:0000269|PubMed:12884090, ECO:0000269|PubMed:16246366, ECO:0000269|PubMed:9987124}; |
| DNA Binding | |
| EC Number | 1.11.1.16 |
| Enzyme Function | FUNCTION: A versatile ligninolytic peroxidase that combines the substrate specificity characteristics of the two other ligninolytic peroxidases, manganese peroxidase and lignin peroxidase. {ECO:0000269|PubMed:9987124}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (10); Chain (1); Disulfide bond (4); Glycosylation (1); Helix (18); Metal binding (13); Mutagenesis (4); Propeptide (1); Region (1); Signal peptide (1); Site (1); Turn (6) |
| Keywords | 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Lignin degradation;Manganese;Metal-binding;Organic radical;Oxidoreductase;Peroxidase;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000269|PubMed:9987124}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (19) |
| Cross Reference PDB | 2BOQ; 2VKA; 2W23; 3FJW; 3FKG; 3FM1; 3FM4; 3FM6; 3FMU; 4FCN; 4FCS; 4FDQ; 4FEF; 4G05; 5ABN; 5ABO; 5ABQ; 5FNB; 5FNE; |
| Mapped Pubmed ID | 18201105; 19158088; 23071108; 26496708; 27118867; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,624 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=19 uM for manganese {ECO:0000269|PubMed:12884090}; KM=3000 uM for veratryl alcohol {ECO:0000269|PubMed:12884090}; KM=4 uM for reactive black 5 {ECO:0000269|PubMed:12884090}; KM=3 uM for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) {ECO:0000269|PubMed:12884090}; |
| Metal Binding | METAL 66; /note=Manganese; /evidence=ECO:0000269|PubMed:16246366; METAL 70; /note=Manganese; /evidence=ECO:0000250; METAL 78; /note=Calcium 1; METAL 90; /note=Calcium 1; via carbonyl oxygen; METAL 92; /note=Calcium 1; METAL 94; /note=Calcium 1; METAL 199; /note=Iron (heme b axial ligand); METAL 200; /note=Calcium 2; METAL 205; /note=Manganese; /evidence=ECO:0000269|PubMed:16246366; METAL 217; /note=Calcium 2; METAL 219; /note=Calcium 2; METAL 222; /note=Calcium 2; via carbonyl oxygen; METAL 224; /note=Calcium 2 |
| Rhea ID | RHEA:22396; RHEA:22776 |
| Cross Reference Brenda | 1.11.1.16;1.11.1.7; |