| IED ID | IndEnz0018000033 |
| Enzyme Type ID | peroxidase000033 |
| Protein Name |
Protein argonaute 4 AtAGO4 Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 |
| Gene Name | AGO4 OCP11 At2g27040 T20P8.9 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDSTNGNGADLESANGANGSGVTEALPPPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLTNHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGEKTLFTYGALPSNKMDFSVVLEEVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKNFRVEISYAAKIPLQALANAMRGQESENSQEAIRVLDIILRQHAARQGCLLVRQSFFHNDPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPYSIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTVADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEKSRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETFPRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEEGNQFRRAPPMIRVENMFKDIQSKLPGVPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVTQCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSPGQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKKNGTEDDGIIKELLVDFYTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHHTKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPISVAQLPRLKDNVANSMFFC |
| Enzyme Length | 924 |
| Uniprot Accession Number | Q9ZVD5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci (PubMed:21738482). Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses (PubMed:12522258, PubMed:14988555, PubMed:15242620, PubMed:16839878, PubMed:16839879, PubMed:16998468, PubMed:17869110, PubMed:17938239, PubMed:17993621, PubMed:18342361, PubMed:18596098, PubMed:19377477, PubMed:20173091). {ECO:0000269|PubMed:12522258, ECO:0000269|PubMed:14988555, ECO:0000269|PubMed:15242620, ECO:0000269|PubMed:16839878, ECO:0000269|PubMed:16839879, ECO:0000269|PubMed:16998468, ECO:0000269|PubMed:17869110, ECO:0000269|PubMed:17938239, ECO:0000269|PubMed:17993621, ECO:0000269|PubMed:18342361, ECO:0000269|PubMed:18596098, ECO:0000269|PubMed:19377477, ECO:0000269|PubMed:20173091, ECO:0000269|PubMed:21738482}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Domain (2); Motif (1); Mutagenesis (4); Region (2) |
| Keywords | Host-virus interaction;Nucleus;Plant defense;RNA-binding;RNA-mediated gene silencing;Reference proteome;Repressor;Ribonucleoprotein;Transcription;Transcription regulation;Translation regulation |
| Interact With | Q9LJF5; Q9M548; Q5D869; Q9LUJ3; F4JW79 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16839878}. Nucleus, nucleoplasm {ECO:0000269|PubMed:19410546}. Nucleus, Cajal body {ECO:0000269|PubMed:16839879, ECO:0000269|PubMed:18266474}. Note=Also located in AB-bodies that are distincts from the Cajal bodies and immediately adjacent to the condensed 45S ribosomal DNA (rDNA) loci (PubMed:18266474). Colocalizes with AGO4 and polymerase V in the nucleoplasm (PubMed:19410546). {ECO:0000269|PubMed:18266474, ECO:0000269|PubMed:19410546}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11016954; 16277745; 17105345; 17332757; 17360559; 17381329; 17412735; 17558406; 17785412; 18022355; 19204117; 19825650; 19834541; 19879665; 19948763; 20410883; 20478993; 20621980; 21070421; 21166475; 21295131; 21379334; 21457371; 21998686; 22242006; 22247253; 22589469; 22608924; 22639648; 22733760; 22771475; 22846193; 23013441; 23104109; 23313553; 23561294; 23847640; 23877244; 24352449; 24493449; 24855943; 24862207; 25100851; 25228471; 25385769; 25491479; 25527293; 25684655; 26023161; 26711010; 27150037; 27243648; 27466226; 27475441; 27531226; 27591749; 27635128; 27659688; 27762650; 27779094; 27813706; 27986858; 28002617; 28046132; 28270624; 28475589; 28783101; 29078035; 29379150; 29525832; 29615117; 30135084; 30692258; 30945177; 31164480; 31274236; 31358683; 31392979; 32460700; |
| Motif | MOTIF 584..591; /note=Nuclear localization signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00768 |
| Gene Encoded By | |
| Mass | 102,840 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |