| IED ID | IndEnz0018000067 |
| Enzyme Type ID | peroxidase000067 |
| Protein Name |
Probable L-ascorbate peroxidase 8, chloroplastic EC 1.11.1.11 OsAPx8 |
| Gene Name | APX8 Os02g0553200 LOC_Os02g34810 P0470G10.5 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAGGLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREILKTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLIQPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGPRVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQSWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTPVPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSPDKPLQSNYFLNIMLLIGGLAFLTSLLGS |
| Enzyme Length | 478 |
| Uniprot Accession Number | Q69SV0 |
| Absorption | |
| Active Site | ACT_SITE 117; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Involved in defense response and tolerance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Plays an important role in hydrogen peroxide removal during infection by Xoo. Involved in response to abiotic stress. Plays a role in hydrogen peroxide removal durings salt stress. {ECO:0000269|PubMed:27185545}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (2); Erroneous gene model prediction (2); Metal binding (4); Region (4); Site (1); Transit peptide (1); Transmembrane (1) |
| Keywords | Calcium;Chloroplast;Heme;Hydrogen peroxide;Iron;Membrane;Metal-binding;Oxidoreductase;Peroxidase;Plant defense;Plastid;Potassium;Reference proteome;Stress response;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Induced by salt stress, abscisic acid (ABA), sodium nitrate and hydrogen peroxide in roots (PubMed:17916638). Induced by infection with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) (PubMed:27185545). Down-regulated by salt stress in leaves (PubMed:16397796). Down-regulated by hydrogen peroxide in leaves (PubMed:25546583). {ECO:0000269|PubMed:16397796, ECO:0000269|PubMed:17916638, ECO:0000269|PubMed:25546583, ECO:0000269|PubMed:27185545}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:27185545, ECO:0000305|PubMed:25546583}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19704658; 21883553; 27716126; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,188 |
| Kinetics | |
| Metal Binding | METAL 246; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 247; /note=Potassium; /evidence=ECO:0000250; METAL 279; /note=Potassium; /evidence=ECO:0000250; METAL 286; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda | 1.11.1.11; |