| IED ID | IndEnz0018000077 |
| Enzyme Type ID | peroxidase000077 |
| Protein Name |
Probable L-ascorbate peroxidase 6, chloroplastic/mitochondrial EC 1.11.1.11 OsAPx6 |
| Gene Name | APX6 Os12g0178100 LOC_Os12g07820 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MAVVHRLLRRGLSAASPLPSLQELGRRPASSSAAAAGDAAAELRGAREDVKQLLKSTSCHPILVRLGWHDAGTYDKNITEWPKCGGANGSLRFEIELKHAANAGLVNALKLIQPIKDKHAGVTYADLFQLASATAIEEAGGPKIPMIYGRVDVAAPEQCPPEGRLPAAGPPSPAEHLREVFYRMGLSDKEIVALSGAHTLGRSRPERSGWGKPETKYTKNGPGAPGGQSWTSQWLKFDNSYFKDIKERRDEDLLVLPTDAVLFEDSSFKIYAEKYAADQDAFFEDYAEAHAKLSNLGAKFDPPKGISLE |
| Enzyme Length | 309 |
| Uniprot Accession Number | P0C0L1 |
| Absorption | |
| Active Site | ACT_SITE 69; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (4); Region (1); Site (1); Transit peptide (1) |
| Keywords | Calcium;Chloroplast;Heme;Hydrogen peroxide;Iron;Metal-binding;Mitochondrion;Oxidoreductase;Peroxidase;Plastid;Potassium;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000305}. Mitochondrion {ECO:0000269|PubMed:16397796}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17916638; 19704658; |
| Motif | |
| Gene Encoded By | |
| Mass | 33,502 |
| Kinetics | |
| Metal Binding | METAL 198; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 199; /note=Potassium; /evidence=ECO:0000250; METAL 231; /note=Potassium; /evidence=ECO:0000250; METAL 238; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda | 1.11.1.11; |