| IED ID | IndEnz0018000078 |
| Enzyme Type ID | peroxidase000078 |
| Protein Name |
Probable L-ascorbate peroxidase 5, chloroplastic EC 1.11.1.11 OsAPx5 |
| Gene Name | APX5 Os12g0178200 LOC_Os12g07830 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MAVVHRILRRGLSAASPLPSLRGLLLVSPQELGRRPASSSSSAAAAAGDVEAELRAAREDVRQLLKSNPCHPILVRLGWHDAGTYDKNITEWPKCGGANGSLRFGVELVHAANKGLLKALFLVIPIKSKYAGVTYADIFQLASATAIEEAGGPKIPMIYGRADVADGEECPPEGRLPAADPPSPAEHLREVFYRMGLSDKEIVALSGAHTLGRARPERSGWGKPETKYTENGPGAPGGQSWTSEWLKFDNSYFKEIKERRDEDLLVLPTDAVLFEDSSFKIHAEKYAEDQDAFFEDYAEAHAKLSNLGAKFDPPKGISLE |
| Enzyme Length | 320 |
| Uniprot Accession Number | P0C0L0 |
| Absorption | |
| Active Site | ACT_SITE 80; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (4); Region (1); Sequence conflict (1); Site (1); Transit peptide (1) |
| Keywords | Calcium;Chloroplast;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Plastid;Potassium;Reference proteome;Transit peptide |
| Interact With | |
| Induction | INDUCTION: Down-regulated by hydrogen peroxide in leaves. {ECO:0000269|PubMed:25546583}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17916638; 19704658; |
| Motif | |
| Gene Encoded By | |
| Mass | 34,759 |
| Kinetics | |
| Metal Binding | METAL 209; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 210; /note=Potassium; /evidence=ECO:0000250; METAL 242; /note=Potassium; /evidence=ECO:0000250; METAL 249; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda | 1.11.1.11; |