IED ID | IndEnz0018000116 |
Enzyme Type ID | peroxidase000116 |
Protein Name |
Alkyl hydroperoxide reductase AhpD EC 1.11.1.28 Alkylhydroperoxidase AhpD |
Gene Name | ahpD Rv2429 MTCY428.17c |
Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Enzyme Sequence | MSIEKLKAALPEYAKDIKLNLSSITRSSVLDQEQLWGTLLASAAATRNPQVLADIGAEATDHLSAAARHAALGAAAIMGMNNVFYRGRGFLEGRYDDLRPGLRMNIIANPGIPKANFELWSFAVSAINGCSHCLVAHEHTLRTVGVDREAIFEALKAAAIVSGVAQALATIEALSPS |
Enzyme Length | 177 |
Uniprot Accession Number | P9WQB5 |
Absorption | |
Active Site | ACT_SITE 130; /note="Proton donor"; /evidence="ECO:0000255|HAMAP-Rule:MF_01676, ECO:0000269|PubMed:11914371"; ACT_SITE 133; /note="Cysteine sulfenic acid (-SOH) intermediate"; /evidence="ECO:0000255|HAMAP-Rule:MF_01676, ECO:0000269|PubMed:11914371" |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=(R)-N(6)-dihydrolipoyl-L-lysyl-[lipoyl-carrier protein] + a hydroperoxide = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + an alcohol + H2O; Xref=Rhea:RHEA:62636, Rhea:RHEA-COMP:10502, Rhea:RHEA-COMP:16355, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:83099, ChEBI:CHEBI:83100; EC=1.11.1.28; Evidence={ECO:0000255|HAMAP-Rule:MF_01676}; |
DNA Binding | |
EC Number | 1.11.1.28 |
Enzyme Function | FUNCTION: Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.; FUNCTION: Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system. |
Temperature Dependency | |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.2. {ECO:0000269|PubMed:12761216}; |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Beta strand (1); Chain (1); Disulfide bond (2); Helix (10); Mutagenesis (7); Turn (2) |
Keywords | 3D-structure;Antioxidant;Disulfide bond;Oxidoreductase;Peroxidase;Redox-active center;Reference proteome |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (4) |
Cross Reference PDB | 1GU9; 1KNC; 1LW1; 1ME5; |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 18,781 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:62636 |
Cross Reference Brenda | 1.11.1.28; |