Detail Information for IndEnz0018000157
IED ID IndEnz0018000157
Enzyme Type ID peroxidase000157
Protein Name Peroxiredoxin Bcp
EC 1.11.1.24
Bacterioferritin comigratory protein
Thioredoxin peroxidase
Thioredoxin-dependent peroxiredoxin Bcp
Gene Name bcp b2480 JW2465
Organism Escherichia coli (strain K12)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12)
Enzyme Sequence MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTPGCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQFGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA
Enzyme Length 156
Uniprot Accession Number P0AE52
Absorption
Active Site ACT_SITE 46; /note="Cysteine sulfenic acid (-SOH) intermediate"; /evidence="ECO:0000269|PubMed:10644761, ECO:0000269|PubMed:19298085"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O; Xref=Rhea:RHEA:62620, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:50058; EC=1.11.1.24; Evidence={ECO:0000269|PubMed:10644761, ECO:0000269|PubMed:21910476};
DNA Binding
EC Number 1.11.1.24
Enzyme Function FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. {ECO:0000269|PubMed:21910476}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (1); Domain (1)
Keywords Antioxidant;Direct protein sequencing;Disulfide bond;Oxidoreductase;Peroxidase;Redox-active center;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16606699; 24561554;
Motif
Gene Encoded By
Mass 17,634
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=76.1 uM for H(2)O(2) (using glutathione Grx1 as electron donor) {ECO:0000269|PubMed:21910476}; KM=99.5 uM for cumene hydroperoxide (using glutathione Grx1 as electron donor) {ECO:0000269|PubMed:21910476}; KM=6800 uM for tert-butyl hydroperoxide (using glutathione Grx1 as electron donor) {ECO:0000269|PubMed:21910476}; KM=14.6 uM for H(2)O(2) (using thioredoxin Trx1 as electron donor) {ECO:0000269|PubMed:21910476}; KM=21.6 uM for cumene hydroperoxide (using thioredoxin Trx1 as electron donor) {ECO:0000269|PubMed:21910476}; KM=574 uM for tert-butyl hydroperoxide (using thioredoxin Trx1 as electron donor) {ECO:0000269|PubMed:21910476};
Metal Binding
Rhea ID RHEA:62620
Cross Reference Brenda 1.11.1.24;