| IED ID | IndEnz0018000214 |
| Enzyme Type ID | peroxidase000214 |
| Protein Name |
Alkyl hydroperoxide reductase C EC 1.11.1.26 Alkyl hydroperoxide reductase protein C22 Peroxiredoxin SCRP-23 Sulfate starvation-induced protein 8 SSI8 Thioredoxin peroxidase |
| Gene Name | ahpC b0605 JW0598 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFTFVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTGALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVASHPGEVCPAKWKEGEATLAPSLDLVGKI |
| Enzyme Length | 187 |
| Uniprot Accession Number | P0AE08 |
| Absorption | |
| Active Site | ACT_SITE 47; /note=Cysteine sulfenic acid (-SOH) intermediate; /evidence=ECO:0000305|PubMed:27892488 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a hydroperoxide + H(+) + NADH = an alcohol + H2O + NAD(+); Xref=Rhea:RHEA:62628, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.11.1.26; Evidence={ECO:0000269|PubMed:11717276}; |
| DNA Binding | |
| EC Number | 1.11.1.26 |
| Enzyme Function | FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Is the primary scavenger for endogenously generated hydrogen peroxides. {ECO:0000269|PubMed:11717276}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (8); Chain (1); Disulfide bond (2); Domain (1); Helix (7); Initiator methionine (1); Modified residue (5); Turn (1) |
| Keywords | 3D-structure;Acetylation;Antioxidant;Cytoplasm;Direct protein sequencing;Disulfide bond;Oxidoreductase;Peroxidase;Redox-active center;Reference proteome |
| Interact With | Itself |
| Induction | INDUCTION: Repressed by sulfate or cysteine. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:7499381}. |
| Modified Residue | MOD_RES 17; /note=N6-acetyllysine; /evidence=ECO:0000269|PubMed:18723842; MOD_RES 93; /note=N6-acetyllysine; /evidence=ECO:0000269|PubMed:18723842; MOD_RES 153; /note=N6-acetyllysine; /evidence=ECO:0000269|PubMed:18723842; MOD_RES 169; /note=N6-acetyllysine; /evidence=ECO:0000269|PubMed:18723842; MOD_RES 171; /note=N6-acetyllysine; /evidence=ECO:0000269|PubMed:18723842 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5B8A; 5B8B; |
| Mapped Pubmed ID | 15690043; 16606699; 16858726; 24561554; |
| Motif | |
| Gene Encoded By | |
| Mass | 20,761 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:62628 |
| Cross Reference Brenda | 1.11.1.26; |