| IED ID | IndEnz0018000215 |
| Enzyme Type ID | peroxidase000215 |
| Protein Name |
Alkyl hydroperoxide reductase C EC 1.11.1.26 Alkyl hydroperoxide reductase protein C22 Peroxiredoxin Thioredoxin peroxidase |
| Gene Name | ahpC STM0608 |
| Organism | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Enzyme Sequence | MSLINTKIKPFKNQAFKNGEFIEVTEKDTEGRWSVFFFYPADFTFVCPTELGDVADHYEELQKLGVDVYSVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTGALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAAHPGEVCPAKWKEGEATLAPSLDLVGKI |
| Enzyme Length | 187 |
| Uniprot Accession Number | P0A251 |
| Absorption | |
| Active Site | ACT_SITE 47; /note=Cysteine sulfenic acid (-SOH) intermediate; /evidence=ECO:0000305|PubMed:18986167 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a hydroperoxide + H(+) + NADH = an alcohol + H2O + NAD(+); Xref=Rhea:RHEA:62628, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.11.1.26; Evidence={ECO:0000269|PubMed:2643600}; |
| DNA Binding | |
| EC Number | 1.11.1.26 |
| Enzyme Function | FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. {ECO:0000269|PubMed:2643600, ECO:0000269|PubMed:8041738}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (10); Chain (1); Disulfide bond (2); Domain (1); Helix (7); Initiator methionine (1); Sequence conflict (6); Turn (3) |
| Keywords | 3D-structure;Antioxidant;Cytoplasm;Direct protein sequencing;Disulfide bond;Oxidoreductase;Peroxidase;Redox-active center;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0AE08}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (15) |
| Cross Reference PDB | 1N8J; 1YEP; 1YEX; 1YF0; 1YF1; 3EMP; 4MA9; 4MAB; 4XRA; 4XRD; 4XS1; 4XS4; 4XS6; 4XTS; 5UKA; |
| Mapped Pubmed ID | 17441733; 18165315; 25633283; 28375740; |
| Motif | |
| Gene Encoded By | |
| Mass | 20,747 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.4 uM for H(2)O(2) {ECO:0000269|PubMed:16060667}; Note=kcat is 55.1 sec(-1) with H(2)O(2) as substrate. {ECO:0000269|PubMed:16060667}; |
| Metal Binding | |
| Rhea ID | RHEA:62628 |
| Cross Reference Brenda | 1.11.1.24;1.11.1.26; |