| IED ID | IndEnz0018000290 |
| Enzyme Type ID | peroxidase000290 |
| Protein Name |
Mannose-1-phosphate guanylyltransferase 1 EC 2.7.7.13 GDP-mannose pyrophosphorylase 1 Protein CYTOKINESIS DEFECTIVE 1 Protein EMBRYO DEFECTIVE 101 Protein HYPERSENSITIVE TO AMMONIUM ION 1 Protein SENSITIVE TO OZONE 1 Protein VITAMIN C DEFECTIVE 1 |
| Gene Name | CYT1 EMB101 GMP1 HSN1 SOZ1 VTC1 At2g39770 T5I7.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKAVGVDEVVLAINYQPEVMLNFLKDFETKLEIKITCSQETEPLGTAGPLALARDKLLDGSGEPFFVLNSDVISEYPLKEMLEFHKSHGGEASIMVTKVDEPSKYGVVVMEESTGRVEKFVEKPKLYVGNKINAGIYLLNPSVLDKIELRPTSIEKETFPKIAAAQGLYAMVLPGFWMDIGQPRDYITGLRLYLDSLRKKSPAKLTSGPHIVGNVLVDETATIGEGCLIGPDVAIGPGCIVESGVRLSRCTVMRGVRIKKHACISSSIIGWHSTVGQWARIENMTILGEDVHVSDEIYSNGGVVLPHKEIKSNILKPEIVM |
| Enzyme Length | 361 |
| Uniprot Accession Number | O22287 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-alpha-D-mannose; Xref=Rhea:RHEA:15229, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57527, ChEBI:CHEBI:58409; EC=2.7.7.13; Evidence={ECO:0000269|PubMed:10097187}; |
| DNA Binding | |
| EC Number | 2.7.7.13 |
| Enzyme Function | FUNCTION: Essential protein during embryogenesis (PubMed:15266054). Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Plays an essential role in plant growth and development and cell-wall architecture. Provides GDP-mannose, used for cell wall carbohydrate biosynthesis, protein N-glycosylation, as well as for the biosynthesis of the antioxidant ascorbate. {ECO:0000269|PubMed:10097187, ECO:0000269|PubMed:10655235, ECO:0000269|PubMed:11226227, ECO:0000269|PubMed:12671089, ECO:0000269|PubMed:15064386, ECO:0000269|PubMed:15266054, ECO:0000269|PubMed:16244149, ECO:0000269|PubMed:16720601, ECO:0000269|PubMed:18849295, ECO:0000269|PubMed:19011088, ECO:0000269|PubMed:8790441, ECO:0000269|PubMed:9390448}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. {ECO:0000269|PubMed:10097187}. |
| nucleotide Binding | |
| Features | Chain (1); Mutagenesis (3); Sequence conflict (2) |
| Keywords | Cytoplasm;GTP-binding;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase |
| Interact With | Q9C9P3 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23424245}. Nucleus {ECO:0000269|PubMed:23424245}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12626114; 12720382; 12874123; 15053760; 15082929; 15665245; 15923322; 16262714; 16263910; 16336779; 16916444; 18775970; 18952318; 19577325; 20121455; 20444215; 20872269; 20955078; 21332510; 21451257; 21631536; 21696975; 21798944; 21926335; 22213815; 22417285; 22995285; 23134520; 23603957; 24088017; 24281833; 24361507; 24393051; 25036484; 25749732; 25954298; 26672069; 27255930; 27561782; 27710946; 27731387; 28082717; 28984358; 29193661; 29929127; 31334862; 33290424; 8264525; 9890811; |
| Motif | |
| Gene Encoded By | |
| Mass | 39,577 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15229 |
| Cross Reference Brenda | 2.7.7.13; |