| IED ID | IndEnz0018000315 |
| Enzyme Type ID | peroxidase000315 |
| Protein Name |
Respiratory burst oxidase homolog protein A EC 1.11.1.- EC 1.6.3.- NADPH oxidase RBOHA AtRBOHA |
| Gene Name | RBOHA At5g07390 T2I1.100 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MMNRSEMQKLGFEHVRYYTESPYNRGESSANVATTSNYYGEDEPYVEITLDIHDDSVSVYGLKSPNHRGAGSNYEDQSLLRQGRSGRSNSVLKRLASSVSTGITRVASSVSSSSARKPPRPQLAKLRRSKSRAELALKGLKFITKTDGVTGWPEVEKRFYVMTMTTNGLLHRSRFGECIGMKSTEFALALFDALARRENVSGDSININELKEFWKQITDQDFDSRLRTFFAMVDKDSDGRLNEAEVREIITLSASANELDNIRRQADEYAALIMEELDPYHYGYIMIENLEILLLQAPMQDVRDGEGKKLSKMLSQNLMVPQSRNLGARFCRGMKYFLFDNWKRVWVMALWIGAMAGLFTWKFMEYRKRSAYEVMGVCVCIAKGAAETLKLNMAMILLPVCRNTITWLRTKTKLSAIVPFDDSLNFHKVIAIGISVGVGIHATSHLACDFPRLIAADEDQYEPMEKYFGPQTKRYLDFVQSVEGVTGIGMVVLMTIAFTLATTWFRRNKLNLPGPLKKITGFNAFWYSHHLFVIVYSLLVVHGFYVYLIIEPWYKKTTWMYLMVPVVLYLCERLIRAFRSSVEAVSVLKVAVLPGNVLSLHLSRPSNFRYKSGQYMYLNCSAVSTLEWHPFSITSAPGDDYLSVHIRVLGDWTKQLRSLFSEVCKPRPPDEHRLNRADSKHWDYIPDFPRILIDGPYGAPAQDYKKFEVVLLVGLGIGATPMISIVSDIINNLKGVEEGSNRRQSPIHNMVTPPVSPSRKSETFRTKRAYFYWVTREQGSFDWFKNVMDEVTETDRKNVIELHNYCTSVYEEGDARSALITMLQSLNHAKHGVDVVSGTRVMSHFARPNWRSVFKRIAVNHPKTRVGVFYCGAAGLVKELRHLSLDFSHKTSTKFIFHKENF |
| Enzyme Length | 902 |
| Uniprot Accession Number | O81209 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.11.1.-; 1.6.3.- |
| Enzyme Function | FUNCTION: Calcium-dependent NADPH oxidase that generates superoxide. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Domain (4); Metal binding (5); Modified residue (2); Region (5); Sequence conflict (3); Topological domain (7); Transmembrane (6) |
| Keywords | Alternative splicing;Calcium;FAD;Flavoprotein;Membrane;Metal-binding;NADP;Oxidoreductase;Peroxidase;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 311; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9FIJ0; MOD_RES 315; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9FIJ0 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12372144; 12773379; 15734912; 15923322; 16362328; 20226671; 30097007; 30570803; |
| Motif | |
| Gene Encoded By | |
| Mass | 102,935 |
| Kinetics | |
| Metal Binding | METAL 234; /note="Calcium"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"; METAL 236; /note="Calcium"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"; METAL 238; /note="Calcium"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"; METAL 240; /note="Calcium; via carbonyl oxygen"; /evidence="ECO:0000250, ECO:0000255|PROSITE-ProRule:PRU00448"; METAL 245; /note="Calcium"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" |
| Rhea ID | |
| Cross Reference Brenda |