| IED ID | IndEnz0018000330 |
| Enzyme Type ID | peroxidase000330 |
| Protein Name |
Putative respiratory burst oxidase homolog protein J EC 1.11.1.- EC 1.6.3.- NADPH oxidase RBOHJ AtRBOHJ |
| Gene Name | RBOHJ At3g45810 F16L2.20 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKNNKKVGTEDSTKWMLESVEIDPKGDSSVKQPESTINSNNPESSGAGGGILKNVSKNLAVGSIIRSMSVNKWRKSGNLGSPSTRKSGNLGPPLPVSQVKRPGPQRVERTTSSAARGLQSLRFLDRTVTGRERDSWRSIENRFNQFAVDGRLPKDKFGVCIGMGDTLEFAAKVYEALGRRRQIKTENGIDKEQLKLFWEDMIKKDLDCRLQIFFDMCDKDGDGKLTEEEVKEVIVLSASANRLVNLKKNAASYASLIMEELDPNEQGYIEMWQLEVLLTGIVSNADSHKVVRKSQQLTRAMIPKRYRTPTSKYVVVTAELMYEHWKKIWVVTLWLAVNVVLFMWKYEEFTTSPLYNITGRCLCAAKGTAEILKLNMALILVPVLRRTLTFLRSTFLNHLIPFDDNINFHKLIAVAIAVISLLHTALHMLCNYPRLSSCPYNFYSDYAGNLLGAKQPTYLGLMLTPVSVTGVLMIIFMGISFTLAMHYFRRNIVKLPIPFNRLAGFNSFWYAHHLLVIAYALLIIHGYILIIEKPWYQKTTWMYVAIPMVLYASERLFSRVQEHNHRVHIIKAIVYSGNVLALYMTKPQGFKYKSGMYMFVKCPDISKFEWHPFSITSAPGDEYLSVHIRALGDWTSELRNRFAETCEPHQKSKPSPNDLIRMETRARGANPHVEESQALFPRIFIKGPYGAPAQSYQKFDILLLIGLGIGATPFISILKDMLNNLKPGIPKTGQKYEGSVGGESLGGSSVYGGSSVNGGGSVNGGGSVSGGGRKFPQRAYFYWVTREQASFEWFKGVMDDIAVYDKTNVIEMHNYLTSMYEAGDARSALIAMVQKLQHAKNGVDIVSESRIRTHFARPNWRKVFSELSNKHETSRIGVFYCGSPTLVRPLKSLCQEFSLESSTRFTFHKENF |
| Enzyme Length | 912 |
| Uniprot Accession Number | Q9LZU9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.11.1.-; 1.6.3.- |
| Enzyme Function | FUNCTION: Calcium-dependent NADPH oxidase that generates superoxide. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (3); Domain (3); Erroneous gene model prediction (1); Metal binding (5); Modified residue (1); Region (4); Topological domain (7); Transmembrane (6) |
| Keywords | Calcium;FAD;Flavoprotein;Membrane;Metal-binding;NADP;Oxidoreductase;Peroxidase;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 294; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9FIJ0 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12372144; 12773379; 16607029; 17409091; 17432890; 18775970; 24302886; 24506280; 24610725; 25751652; 29242232; 30097007; |
| Motif | |
| Gene Encoded By | |
| Mass | 102,937 |
| Kinetics | |
| Metal Binding | METAL 218; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 220; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 222; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 224; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 229; /note=Calcium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| Rhea ID | |
| Cross Reference Brenda |