Detail Information for IndEnz0018000354
IED ID IndEnz0018000354
Enzyme Type ID peroxidase000354
Protein Name Glutathione S-transferase F8, chloroplastic
AtGSTF8
EC 2.5.1.18
AtGSTF5
GST class-phi member 8
Glutathione S-transferase 6
Gene Name GSTF8 GST6 GSTF5 At2g47730 F17A22.12
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGAIQARLPLFLSPPSIKHHTFLHSSSSNSNFKIRSNKSSSSSSSSIIMASIKVHGVPMSTATMRVLATLYEKDLQFELIPVDMRAGAHKQEAHLALNPFGQIPALEDGDLTLFESRAITQYLAEEYSEKGEKLISQDCKKVKATTNVWLQVEGQQFDPNASKLAFERVFKGMFGMTTDPAAVQELEGKLQKVLDVYEARLAKSEFLAGDSFTLADLHHLPAIHYLLGTDSKVLFDSRPKVSEWIKKISARPAWAKVIDLQKQ
Enzyme Length 263
Uniprot Accession Number Q96266
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18;
DNA Binding
EC Number 2.5.1.18
Enzyme Function FUNCTION: In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. {ECO:0000269|PubMed:12090627}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (1); Chain (1); Domain (2); Erroneous gene model prediction (1); Modified residue (1); Region (4); Transit peptide (1)
Keywords Alternative splicing;Chloroplast;Cytoplasm;Detoxification;Oxidoreductase;Peroxidase;Phosphoprotein;Plastid;Reference proteome;Stress response;Transferase;Transit peptide
Interact With
Induction INDUCTION: By salicylic acid, ethylene, methyl jasmonate, auxin, H(2)O(2), metolachlor, and the pathogen Hyaloperonospora parasitica. {ECO:0000269|PubMed:12090627, ECO:0000269|PubMed:16829588, ECO:0000269|PubMed:9011080}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000269|PubMed:17670748}. Cytoplasm, cytosol {ECO:0000269|PubMed:17670748}.
Modified Residue MOD_RES 177; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:22092075
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10571852; 10785664; 11862948; 12912986; 12938931; 14535880; 14617066; 15159623; 15276439; 15352244; 16242667; 16247729; 16648217; 16923014; 17028151; 17151019; 17916636; 18334669; 18431481; 18541338; 18633119; 18775970; 18848650; 18849295; 19174456; 20351290; 20405473; 21670306; 22476218; 22633844; 23083132; 25985302; 28627464; 28782990; 30862010; 30925335; 31128707; 31519798; 31863837;
Motif
Gene Encoded By
Mass 29,232
Kinetics
Metal Binding
Rhea ID RHEA:16437
Cross Reference Brenda