| IED ID | IndEnz0018000354 |
| Enzyme Type ID | peroxidase000354 |
| Protein Name |
Glutathione S-transferase F8, chloroplastic AtGSTF8 EC 2.5.1.18 AtGSTF5 GST class-phi member 8 Glutathione S-transferase 6 |
| Gene Name | GSTF8 GST6 GSTF5 At2g47730 F17A22.12 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGAIQARLPLFLSPPSIKHHTFLHSSSSNSNFKIRSNKSSSSSSSSIIMASIKVHGVPMSTATMRVLATLYEKDLQFELIPVDMRAGAHKQEAHLALNPFGQIPALEDGDLTLFESRAITQYLAEEYSEKGEKLISQDCKKVKATTNVWLQVEGQQFDPNASKLAFERVFKGMFGMTTDPAAVQELEGKLQKVLDVYEARLAKSEFLAGDSFTLADLHHLPAIHYLLGTDSKVLFDSRPKVSEWIKKISARPAWAKVIDLQKQ |
| Enzyme Length | 263 |
| Uniprot Accession Number | Q96266 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; |
| DNA Binding | |
| EC Number | 2.5.1.18 |
| Enzyme Function | FUNCTION: In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. {ECO:0000269|PubMed:12090627}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Domain (2); Erroneous gene model prediction (1); Modified residue (1); Region (4); Transit peptide (1) |
| Keywords | Alternative splicing;Chloroplast;Cytoplasm;Detoxification;Oxidoreductase;Peroxidase;Phosphoprotein;Plastid;Reference proteome;Stress response;Transferase;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By salicylic acid, ethylene, methyl jasmonate, auxin, H(2)O(2), metolachlor, and the pathogen Hyaloperonospora parasitica. {ECO:0000269|PubMed:12090627, ECO:0000269|PubMed:16829588, ECO:0000269|PubMed:9011080}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000269|PubMed:17670748}. Cytoplasm, cytosol {ECO:0000269|PubMed:17670748}. |
| Modified Residue | MOD_RES 177; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:22092075 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10571852; 10785664; 11862948; 12912986; 12938931; 14535880; 14617066; 15159623; 15276439; 15352244; 16242667; 16247729; 16648217; 16923014; 17028151; 17151019; 17916636; 18334669; 18431481; 18541338; 18633119; 18775970; 18848650; 18849295; 19174456; 20351290; 20405473; 21670306; 22476218; 22633844; 23083132; 25985302; 28627464; 28782990; 30862010; 30925335; 31128707; 31519798; 31863837; |
| Motif | |
| Gene Encoded By | |
| Mass | 29,232 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:16437 |
| Cross Reference Brenda |